江西柿属种质资源遗传多样性的IRAP标记分析

利用逆转座子间扩增多态性（Inter-retrotransposon Amplified Polymorphism,IRAP）分子标记对收集的49份江西柿属种质资源进行了遗传多样性分析。从26个IRAP引物中筛选出扩增条带清晰且多态性丰富的10个引物,PCR扩增产物经聚丙烯酰胺凝胶电泳（PAGE）得到清晰的指纹图谱。将418条清晰条带（多态性100%）转换为0/1矩阵,聚类分析结果表明：供试的江西柿属种质资源的遗传相似系数在0.69~0.92之间,分属柿、油柿、野柿三个种,遗传多样性丰富;明晰了几个分类关系模糊的柿种质资源的种属关系。...

Full description

Saved in:
Bibliographic Details
Published in江西农业学报 Vol. 29; no. 6; pp. 29 - 34
Main Author 曾明 杜晓云 吴美华 王彦波
Format Journal Article
LanguageChinese
Published 江西省农业科学院园艺研究所,江西南昌,330200 2017
Subjects
IRAP
柿属
江西
种质资源
遗传多样性
柿属
遗传多样性
种质资源
Jiangxi
Diospyros
IRAP
Genetic diversity
江西
Germplasm resource
Online AccessGet full text

Cover

More Information
Summary:利用逆转座子间扩增多态性（Inter-retrotransposon Amplified Polymorphism,IRAP）分子标记对收集的49份江西柿属种质资源进行了遗传多样性分析。从26个IRAP引物中筛选出扩增条带清晰且多态性丰富的10个引物,PCR扩增产物经聚丙烯酰胺凝胶电泳（PAGE）得到清晰的指纹图谱。将418条清晰条带（多态性100%）转换为0/1矩阵,聚类分析结果表明：供试的江西柿属种质资源的遗传相似系数在0.69~0.92之间,分属柿、油柿、野柿三个种,遗传多样性丰富;明晰了几个分类关系模糊的柿种质资源的种属关系。
Bibliography:Jiangxi ; Diospyros ; Germplasm resource ; Genetic diversity ; IRAP
36-1124/S
The genetic diversities of 49 germplasm resources in Diospyros in Jiangxi were analyzed by using IRAP （ Inter-ret- rotransposon Amplified Polymorphism） molecular marker. Ten primers which had clear bands and polymorphism in PCR amplifi- cation were screened out from 26 IRAP primers, and the PCR-amplified products presented clear fingerprint by polyacrylamide gel electrophoresis （PAGE）. The 418 clear bands （100% diversity） were transformed into 0/1 matrix, and the clustering analysis showed that the genetic similarity coefficients among the tested 49 germplasm resources varied from 0.69 to 0.92, and these germplasm resources belonged to 3 species （ Diospyros kaki Thunb., Diospyros oleifera Cheng, and Diospyros kaki var. silves- tis Makino）, which revealed abundant genetic diversity. In addition, the species taxonomic relationships among several Diospyros spp. germplasm resources were clarified.
ZENG Ming, DU Xiao-yun , WU Mei-hua, WANG
ISSN:1001-8581
DOI:10.19386/j.cnki.jxnyxb.2017.06.07