一株化血胆组织培养污染内生细菌的分离鉴定及防治

采用NA培养基分离纯化细菌，通过形态特征、生理生化指标结合16SrDNA序列同源性分析，对引起化血胆组培苗污染的内生细菌进行鉴定。结果表明，引起化血胆组培苗污染的内生细菌为HXD5菌株，其形态特征及22项生理生化指标与枯草芽孢杆菌相符；16SrDNA序列分析发现，HXD5与最酬幻蚓‰（HQ727971）、B.vallismortis（FJ386541）、B．lichenlformis（EF644414）、B．subtilis（AY583216）在同一系统发育分支，同源性为99．9％-100％。因此确定引起化血胆组培苗污染的内生细菌HXD5为枯草芽孢杆菌。抑菌试验表明，氯霉素对HXD5的抑菌效果...

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Published in西南农业学报 Vol. 29; no. 7; pp. 1707 - 1712
Main Author 赵凤 耿开友 胡昳 夏体渊 陈泽斌 任稹 靳松
Format Journal Article
LanguageChinese
Published 昆明学院科研处,云南昆明,650214%昆明学院农学院,云南昆明,650214 2016
Subjects
内生细菌
分离
化血胆
组织培养
鉴定
防治
Control
组织培养
化血胆
鉴定
Melasma arvense
内生细菌
Tissue culture
Isolation
Endophytic bacteria
Identification
防治
分离
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ISSN1001-4829
DOI10.16213/j.cnki.scjas.2016.07.037

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Summary:采用NA培养基分离纯化细菌，通过形态特征、生理生化指标结合16SrDNA序列同源性分析，对引起化血胆组培苗污染的内生细菌进行鉴定。结果表明，引起化血胆组培苗污染的内生细菌为HXD5菌株，其形态特征及22项生理生化指标与枯草芽孢杆菌相符；16SrDNA序列分析发现，HXD5与最酬幻蚓‰（HQ727971）、B.vallismortis（FJ386541）、B．lichenlformis（EF644414）、B．subtilis（AY583216）在同一系统发育分支，同源性为99．9％-100％。因此确定引起化血胆组培苗污染的内生细菌HXD5为枯草芽孢杆菌。抑菌试验表明，氯霉素对HXD5的抑菌效果要优于链霉素。
Bibliography:Using the NA culture medium to isolate the strain, the bacterial species were identified by morphology, physiological and biochemical and the sequence analysis of the 16S rDNA. The results from physiological and biochemical tests showed that the morphological charac-teristics of the strain HXD5 matched the descriptions of Bacillus subtilis. The sequence analysis of 16S rDNA indicated that this strain bad the same embranchment with the B. amyloliquefaciens （HQ727971）, B. vallismortis （FJ386541）, B. licheniformis （EF644414）, B. subtilis （AY583216） in the phylogenetic tree with the homology of 99.9 % - 100 %. The HXD5 strain was identified as Bacillus subtilis. The tests of bacterial inhibition in vitro showed that the chloromycetin had stronger antimicrobial effect than streptomycin.
Melasma arvense; Tissue culture; Endophytic bacteria; Isolation; Identification; Control
51-1213/S
ZHAO Feng , GENG Kai-you , HU Yi, XIA Ti-yuan, CHEN Ze-bin, BEN Zhen , JlN Song （1. Scientific Research Department, Kunming University,
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2016.07.037