Cell-associated oligosaccharides of Bradyrhizobium spp

• Published in: Journal of Bacteriology Vol. 172; no. 1; pp. 136 - 142
• Main Authors: Miller, K.J. (The Pennsylvania State University, University Park, PA), Gore, R.S, Johnson, R, Benesi, A.J, Reinhold, V.N
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.01.1990
• Subjects: AGROBACTERIUM; AZUCARES; Bacteriology; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; GLUCANE; GLUCANO; Glucans - analysis; Magnetic Resonance Spectroscopy; Microbiology; Morphology, structure, chemical composition; Oligosaccharides - analysis; RHIZOBIACEAE; Rhizobiaceae - analysis; RHIZOBIUM; Rhizobium - analysis; SUCRES; Characterization; Glucan; Oligosaccharide; FAB mass spectrum; Bacteria; Glucosidic bond; Chemical composition; NMR spectrometry; Rhizobiaceae; Glucose; Bradyrhizobium; Supercritical fluid chromatography
• ISSN: 0021-9193; 1098-5530; 1067-8832
• DOI: 10.1128/jb.172.1.136-142.1990

We report the initial characterization of the cell-associated oligosaccharides produced by four Bradyrhizobium strains: Bradyrhizobium japonicum USDA 110, USDA 94, and ATCC 10324 and Bradyrhizobium sp. strain 32H1. The cell-associated oligosaccharides of these strains were found to be composed solely of glucose and were predominantly smaller than the cyclic beta-1,2-glucans produced by Agrobacterium and Rhizobium species. Linkage studies and nuclear magnetic resonance analyses demonstrated that the bradyrhizobial glucans are linked primarily by beta-1,6 and beta-1,3 glycosidic bonds. Thus, the bradyrhizobia appear to synthesize cell-associated oligosaccharides of structural character substantially different from that of the cyclic beta-1,2-glucans produced by Agrobacterium and Rhizobium species