Apoptosis of proliferative cortical tubular epithelia in chronic progressive nephrosis of rats

• Published in: Journal of Veterinary Medical Science Vol. 62; no. 4; pp. 367 - 374
• Main Authors: Ikeyama, S. (Nippon Soda Co. Ltd., Odawara, Kanagawa (Japan)), Nukui, T, Nishibe, T, Furukawa, S, Goryo, M, Okada, K
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.04.2000; Japan Science and Technology Agency
• Subjects: ADN; Animals; APOPTOSE; APOPTOSIS; DNA; ENFERMEDADES RENALES; In Situ Nick-End Labeling; Kidney Cortex - pathology; KIDNEY DISEASES; Kidney Glomerulus - pathology; Kidney Tubules - pathology; Male; NEPHROPATHIE; nephrosis; Nephrosis - pathology; Nephrosis - veterinary; PCNA; Proliferating Cell Nuclear Antigen - analysis; RAT; RATA; RATS; Rats, Inbred F344; Rodent Diseases - pathology; TUNEL
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.62.367

To investigate the role of apoptosis in the pathogenesis of rat chronic progressive nephrosis (CPN), the kidney of male F344/DuCrj rats, 19, 59, and 111 weeks of age, was examined histologically. In situ analysis for DNA fragmentation and proliferating cell nuclear antigen (PCNA) was performed simultaneously by TdT-mediated dUTP-biotin nick end labeling (TUNEL) and immunohistochemistry, respectively. CPN was seen in all the kidneys of 59-week-old (n=6) and Ill-week-old rats (n=16), correlating significantly (p0.01) with age. There were apoptotic bodies (ABs) in the single-layered epithelia of dilated tubules (SLD) and the multilayered epithelia (ML) of the cortical tubules. There were no ABs in any of the kidneys of the 19-week-old (n