2-(5-Hydrazinocarbonyl-2-furyl)-5, 6-dimethoxybenzothiazole as a Precolumn Fluorescence Derivatization Reagent for Carboxylic Acids in High-Performance Liquid Chromatography and Its Application to the Assay of Fatty Acids in Human Serum

A precolumn fluorescence derivatization reagent for carboxylic acids, 2-(5-hydrazinocarbonyl-2-furyl)-5, 6-dimethoxybenzothiazole, was developed and its applicability to fatty acids in biological samples was examined. This reagent was proved to be useful in the determination of fatty acids in high-p...

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Published inAnalytical Sciences Vol. 11; no. 1; pp. 103 - 107
Main Authors SAITO, Mikihiko, USHIJIMA, Tamano, SASAMOTO, Kazumi, OHKURA, Yosuke, UENO, Keiyu
Format Journal Article
LanguageEnglish
Published Tokyo The Japan Society for Analytical Chemistry 1995
Japan Society for Analytical Chemistry
Japan Science and Technology Agency
Subjects
acid hydrazide
Analytical chemistry
benzothiazole
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
fatty acid
Fluorescence derivatization reagent
high-performance liquid chromatography
Other chromatographic methods
serum
Biological fluid
Chemical analysis
Precolumn
HPLC chromatography
Serum
Derivatization
Fatty acids
Fluorescence spectrometry
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Summary:A precolumn fluorescence derivatization reagent for carboxylic acids, 2-(5-hydrazinocarbonyl-2-furyl)-5, 6-dimethoxybenzothiazole, was developed and its applicability to fatty acids in biological samples was examined. This reagent was proved to be useful in the determination of fatty acids in high-performance liquid chromatography with the detection limit of approximately 50fmol per 10-μl injection volume. Fatty acids in human serum were assayed by the derivatization method using the reagent. The method was also applied to some prostaglandins.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.11.103