Genome editing methods in animal models

• Published in: Animal cells and systems Vol. 24; no. 1; pp. 8 - 16
• Main Authors: Lee, Hyunji, Yoon, Da Eun, Kim, Kyoungmi
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02.01.2020; Taylor & Francis Ltd; Taylor & Francis Group; 한국통합생물학회
• Subjects: Animal diseases; animal model; Animal models; CRISPR; CRISPR-Cas9 system; Deoxyribonucleic acid; DNA; Editing; Embryo cells; Gene sequencing; Genetic engineering; Genome editing; Genomes; In vivo delivery; In vivo methods and tests; Mutation; Neurobiology & Physiology; Nucleotide sequence; Stem cell transplantation; Stem cells; Therapeutic applications; 생물학; genome editing; animal model; In vivo delivery; CRISPR-Cas9 system
• ISSN: 1976-8354; 2151-2485
• DOI: 10.1080/19768354.2020.1726462

Genetically engineered animal models that reproduce human diseases are very important for the pathological study of various conditions. The development of the clustered regularly interspaced short palindromic repeats (CRISPR) system has enabled a faster and cheaper production of animal models compared with traditional gene-targeting methods using embryonic stem cells. Genome editing tools based on the CRISPR-Cas9 system are a breakthrough technology that allows the precise introduction of mutations at the target DNA sequences. In particular, this accelerated the creation of animal models, and greatly contributed to the research that utilized them. In this review, we introduce various strategies based on the CRISPR-Cas9 system for building animal models of human diseases and describe various in vivo delivery methods of CRISPR-Cas9 that are applied to disease models for therapeutic purposes. In addition, we summarize the currently available animal models of human diseases that were generated using the CRISPR-Cas9 system and discuss future directions.