Identification of Newly Synthesized Proteins by Echinococcus granulosus Protoscoleces upon Induction of Strobilation

• Published in: PLoS neglected tropical diseases Vol. 9; no. 9; p. e0004085
• Main Authors: Debarba, João Antonio, Monteiro, Karina Mariante, Moura, Hercules, Barr, John R, Ferreira, Henrique Bunselmeyer, Zaha, Arnaldo
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.09.2015; Public Library of Science (PLoS)
• Subjects: Abattoirs; Animals; Cattle; Cattle Diseases - parasitology; Chromatography, Liquid; Cysts; Disease; Echinococcosis - parasitology; Echinococcosis - veterinary; Echinococcus granulosus - chemistry; Echinococcus granulosus - physiology; Electrophoresis, Polyacrylamide Gel; Experiments; Gene expression; Gene Expression Profiling; Genetic aspects; Genetic research; Helminth Proteins - analysis; Labeling; Laboratories; Life Cycle Stages; Mass spectrometry; Metabolism; Microscopy; Microscopy, Confocal; Observations; Parasites; Protein biosynthesis; Proteins; Reproduction, Asexual; Scientific imaging; Studies; Tandem Mass Spectrometry; Tropical diseases; Visualization
• ISSN: 1935-2735; 1935-2727; 1935-2735
• DOI: 10.1371/journal.pntd.0004085

The proteins responsible for the key molecular events leading to the structural changes between the developmental stages of Echinococcus granulosus remain unknown. In this work, azidohomoalanine (AHA)-specific labeling was used to identify proteins expressed by E. granulosus protoscoleces (PSCs) upon the induction of strobilar development. The in vitro incorporation of AHA with different tags into newly synthesized proteins (NSPs) by PSCs was analyzed using SDS-PAGE and confocal microscopy. The LC-MS/MS analysis of AHA-labeled NSPs by PSCs undergoing strobilation allowed for the identification of 365 proteins, of which 75 were differentially expressed in comparison between the presence or absence of strobilation stimuli and 51 were expressed exclusively in either condition. These proteins were mainly involved in metabolic, regulatory and signaling processes. After the controlled-labeling of proteins during the induction of strobilar development, we identified modifications in protein expression. The changes in the metabolism and the activation of control and signaling pathways may be important for the correct parasite development and be target for further studies.