Xylanases and carboxymethylcellulases of the rumen protozoa Polyplastron multivesiculatum, Eudiplodinium maggii and Entodinium sp

• Published in: FEMS microbiology letters Vol. 244; no. 1; pp. 149 - 156
• Main Authors: Béra-Maillet, Christel, Devillard, Estelle, Cezette, Magalie, Jouany, Jean-Pierre, Forano, Evelyne
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 01.03.2005; Blackwell Publishing Ltd; Blackwell; Oxford University Press; Wiley-Blackwell
• Subjects: Animals; Biochemistry. Physiology. Immunology. Molecular biology; Biological and medical sciences; Carboxymethylcellulase; Cattle; Cellulase - genetics; Cellulase - metabolism; Ciliates; Ciliophora - enzymology; Ciliophora - genetics; Computer Science; Endo-1,4-beta Xylanases - genetics; Endo-1,4-beta Xylanases - metabolism; endo-1,4-beta-glucanase; Endoglucanase; Entodinium; Entodinium sp; enzyme activity; Eudiplodinium; Eudiplodinium maggii; Fundamental and applied biological sciences. Psychology; Genes, Protozoan; Life Sciences; Microbiology; Phylogeny; Polyplastron multivesiculatum; Polysaccharides; Protozoa; Rumen; Rumen - parasitology; rumen protozoa; Saccharides; Sheep; Species Specificity; structural genes; Xylanase; xylanases; xyn11D gene; Rumen; Eudiplodinium maggii; Carboxymethylcellulase; Entodinium sp; Xylanase; Polyplastron multivesiculatum; sp; Molecular arrangement; Protozoa; Enzyme; Phylogenetic tree; Genetic diversity; Glycosidases; Hydrolases; Xylan endo-1,3-β-xylosidase; O-Glycosidases; carboxymethylcellulase; rumen; xylanase
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/j.femsle.2005.01.035

Endoglucanase and xylanase activities of three rumen protozoa, Polyplastron multivesiculatum, Eudiplodinium maggii, and Entodinium sp. were compared qualitatively by zymograms and quantitatively by measuring specific activities against different polysaccharides. A set of carboxymethylcellulases and xylanases was produced by the large ciliates whereas no band of activity was observed for Entodinium sp. in zymograms. Specific activity of endoglucanases from P. multivesiculatum (1.3 μmol mg prot −1 min −1) was twice that of E. maggii, whereas xylanase specific activity (4.5 μmol mg prot −1 min −1) was only half. Very weak activities were observed for Entodinium sp. A new xylanase gene, xyn11D, from P. multivesiculatum was reported and its gene product compared to 33 other family 11 xylanases. Phylogenetic analysis showed that xylanase sequences from rumen protozoa are closely related to those of bacteria.