N‐glycan on Asn54 affects the atypical N‐glycan composition of plant‐produced interleukin‐22, but does not influence its activity

• Published in: Plant biotechnology journal Vol. 14; no. 2; pp. 670 - 681
• Main Authors: Wilbers, Ruud H. P, Westerhof, Lotte B, Reuter, Lauri J, Castilho, Alexandra, Raaij, Debbie R, Nguyen, Dieu‐Linh, Lozano‐Torres, Jose L, Smant, Geert, Hokke, Cornelis H, Bakker, Jaap, Schots, Arjen
• Format: Journal Article
• Language: English
• Published: England Blackwell Pub 01.02.2016; John Wiley & Sons, Inc
• Subjects: Aggregates; Analysis; Animals; antimicrobial proteins; Asparagine - metabolism; bioactive properties; Biological; Biological activity; Biotechnology; Cell differentiation; Cell proliferation; Composition; core α1,6‐fucose; Cytokines; Deglycosylation; Drosophila melanogaster; engineering; Epithelial cells; EPS-2; Folding; Glycan; Glycoproteins; Glycosylation; glyco‐engineering; Health aspects; HEK293 Cells; Human; Humans; Immune system; Interleukin; Interleukin-22; Interleukins; Interleukins - biosynthesis; Interleukins - isolation & purification; Interleukins - metabolism; Kinases; Laboratorium voor Nematologie; Laboratory of Nematology; Lewis A; Metabolic Engineering; mice; Nicotiana; Nicotiana - genetics; Nicotiana - metabolism; Nicotiana benthamiana; N‐glycosylation; Plant Leaves - metabolism; Plants (organisms); Plants, Genetically Modified; Polysaccharides; Polysaccharides - metabolism; Protein folding; protein synthesis; Proteins; Site-directed mutagenesis; Netherlands; Lewis A; glyco-engineering; core α1,6-fucose; Nicotiana; interleukin-22; N-glycosylation
• ISSN: 1467-7644; 1467-7652
• DOI: 10.1111/pbi.12414

Human interleukin‐22 (IL‐22) is a member of the IL‐10 cytokine family that has recently been shown to have major therapeutic potential. IL‐22 is an unusual cytokine as it does not act directly on immune cells. Instead, IL‐22 controls the differentiation, proliferation and antimicrobial protein expression of epithelial cells, thereby maintaining epithelial barrier function. In this study, we transiently expressed human IL‐22 in Nicotiana benthamiana plants and investigated the role of N‐glycosylation on protein folding and biological activity. Expression levels of IL‐22 were up to 5.4 μg/mg TSP, and N‐glycan analysis revealed the presence of the atypical Lewis A structure. Surprisingly, upon engineering of human‐like N‐glycans on IL‐22 by co‐expressing mouse FUT8 in ΔXT/FT plants a strong reduction in Lewis A was observed. Also, core α1,6‐fucoylation did not improve the biological activity of IL‐22. The combination of site‐directed mutagenesis of Asn54 and in vivo deglycosylation with PNGase F also revealed that N‐glycosylation at this position is not required for proper protein folding. However, we do show that the presence of a N‐glycan on Asn54 contributes to the atypical N‐glycan composition of plant‐produced IL‐22 and influences the N‐glycan composition of N‐glycans on other positions. Altogether, our data demonstrate that plants offer an excellent tool to investigate the role of N‐glycosylation on folding and activity of recombinant glycoproteins, such as IL‐22.