Analyte zone sharpening in pressurized capillary electrochromatography based on electrophoretic migration under a heterogeneous field
Significant peak width reductions, or peak height enhancements, of angiotensins were observed when a high voltage was applied to hydrophilic interaction pressurized capillary electrochromatography using gradient elution with mobile phases containing perchloric acid. The investigation using a contact...
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Published in | Journal of separation science Vol. 37; no. 21; pp. 3181 - 3187 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
Wiley-VCH
01.11.2014
Blackwell Publishing Ltd Wiley Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
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Summary: | Significant peak width reductions, or peak height enhancements, of angiotensins were observed when a high voltage was applied to hydrophilic interaction pressurized capillary electrochromatography using gradient elution with mobile phases containing perchloric acid. The investigation using a contactless conductivity detector revealed that perchloric acid was adsorbed on the surface of the stationary phase, when the acetonitrile content in the mobile phase was high, and released from the stationary phase by increasing the water content during a gradient procedure. The released perchloric acid formed a highly concentrated zone moving from the column inlet to the outlet. The electrochromatographic behavior of the analytes, primarily electrophoretic migration, was changed in this zone. As a consequence of the significant variation in migration velocity of the analytes, the sample band width was reduced similar fashion to on‐capillary concentration in capillary electrophoresis. Using this result, the reduction of band width and enhancement in separation efficiency was demonstrated in reversed‐phase pressurized electrochromatography, in which the conductivity of the mobile phase was significantly altered using a step gradient. The resolution between benzoic acid and 1‐naphthalene sulfonic acid was successfully improved from 2.7 to 4.3 by using the band width reduction method based on field‐amplified stacking. |
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Bibliography: | http://dx.doi.org/10.1002/jssc.201400668 This paper is included in the virtual special issue on Amino acids, proteins and peptides available at the Journal of Separation Science website. ArticleID:JSSC3913 ark:/67375/WNG-S3L9NR67-2 istex:0D25E31A14D20B75EFB014DDD660456822FBA7D4 Amino acids, proteins and peptides This paper is included in the virtual special issue on available at the Journal of Separation Science website. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1615-9306 1615-9314 |
DOI: | 10.1002/jssc.201400668 |