姬菇258和金凤2-1杂交构建姬菇新菌株

以姬菇258和金凤2-1为亲本,单孢杂交,获得杂交菌株81株。通过定性出菇表明,其中44号、6号、115号和7号菌株子实体形态具有姬菇的典型特征。通过拮抗反应、ISSR分子标记及聚类分析表明,这4株姬菇新菌株与亲本之间存在遗传差异,证实了杂交姬菇新菌株的真实性;4株新菌株之间也各自保持着个体遗传上的独立和稳定,即构建创制出了4株杂交姬菇新菌株。...

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Published in西南农业学报 Vol. 28; no. 6; pp. 2655 - 2660
Main Author 谭伟 周洁 曹雪莲 苗人云 贾定洪 黄忠乾 甘炳成 彭卫红 郑林用
Format Journal Article
LanguageChinese
Published 四川省农业科学院土壤肥料研究所,四川成都,610066%四川金地菌类有限责任公司,四川成都,610066 2015
Subjects
ISSR标记
出菇试验
单孢杂交
姬菇258
姬菇特征
拮抗反应
聚类分析
金凤2-1
Cluster analysis
Pleurotus sp.Jin feng2-1
P.cornucopiae No.258
Monosporous
拮抗反应
ISSR标记
出菇试验
金凤2-1
单孢杂交
姬菇258
ISSR
Antagonism
Fruiting test
聚类分析
姬菇特征
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Summary:以姬菇258和金凤2-1为亲本,单孢杂交,获得杂交菌株81株。通过定性出菇表明,其中44号、6号、115号和7号菌株子实体形态具有姬菇的典型特征。通过拮抗反应、ISSR分子标记及聚类分析表明,这4株姬菇新菌株与亲本之间存在遗传差异,证实了杂交姬菇新菌株的真实性;4株新菌株之间也各自保持着个体遗传上的独立和稳定,即构建创制出了4株杂交姬菇新菌株。
Bibliography:TAN Wei;ZHOU Jie;CAO Xue-lian;MIAO Ren-yun;JIA Ding-hong;HUANG Zhong-qian;GAN Bing-cheng;PENG Wei-hong;ZHENG Lin-yong（1. Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Sichuan Chengdu 610066, China, 2. Sichuan Jindi Fungus Co., Ltd, Siehuan Chengdu 610066, China）
51-1213/S
P.cornucopiae No.258; Pleurotus sp.Jin feng2-1; Monosporous; Fruiting test; Antagonism; ISSR; Cluster analysis
81 hybrid strains were obtained by single spore crossbreeding between Pleurotus cornucopiae No. 258 and Pleurotus sp. Jin feng2-1. The fruiting test showed that No. 44,No. 6,No. 115 and No. 7 strains＇ fruiting bodies had the characteristic feature of P. cornucopiae.Antagonistic test,ISSR marker test and cluster analysis showed that the four hybrid strains＇ genetic were different from parents and they were different from each other.
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2015.06.056