龙荔基因组DNA的提取及ISSR-PCR体系的建立与优化
应用改良的CTAB法提取龙荔基因组DNA,探讨龙荔ISSR-PCR扩增反应体系的建立和优化。结果表明,经过改良的CTAB法提取龙荔基因组DNA效果良好,所提取的DNA纯度较高,符合ISSR-PCR反应的要求;龙荔ISSR-PCR最佳反应体系（反应总体积20μl）：1×Buffer缓冲液（含适量Mg^2＋）,1 UTaqDNA聚合酶,0.20 mmol/L dNTP,0.25μmol/L引物,DNA模板70-80 ng/μl,退火温度53-54℃。...
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| Published in | Xinan nongye xuebao Vol. 22; no. 1; pp. 145 - 149 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
广西大学农学院,广西,南宁530005%广西农业科学院园艺研究所,广西,南宁530007
2009
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-4829 |
| DOI | 10.3969/j.issn.1001-4829.2009.01.036 |
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| Abstract | 应用改良的CTAB法提取龙荔基因组DNA,探讨龙荔ISSR-PCR扩增反应体系的建立和优化。结果表明,经过改良的CTAB法提取龙荔基因组DNA效果良好,所提取的DNA纯度较高,符合ISSR-PCR反应的要求;龙荔ISSR-PCR最佳反应体系（反应总体积20μl）：1×Buffer缓冲液（含适量Mg^2＋）,1 UTaqDNA聚合酶,0.20 mmol/L dNTP,0.25μmol/L引物,DNA模板70-80 ng/μl,退火温度53-54℃。 |
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| AbstractList | S667.1; 应用改良的CTAB法提取龙荔基因组DNA,探讨龙荔ISSR-PCR扩增反应体系的建立和优化.结果表明,经过改良的CTAB法提取龙荔基因组DNA效果良好,所提取的DNA纯度较高,符合ISSR-PCR反应的要求;龙荔ISSR-PCR最佳反应体系(反应总体积20 μl):1 × Buffer缓冲液(含适量Mg2+),1 U Taq DNA聚合酶,0.20 mmol/L dNTP,0.25 μmol/L引物,DNA模板70 ～ 80 ng/μl,退火温度53 ～ 54 ℃. 应用改良的CTAB法提取龙荔基因组DNA,探讨龙荔ISSR-PCR扩增反应体系的建立和优化。结果表明,经过改良的CTAB法提取龙荔基因组DNA效果良好,所提取的DNA纯度较高,符合ISSR-PCR反应的要求;龙荔ISSR-PCR最佳反应体系（反应总体积20μl）：1×Buffer缓冲液（含适量Mg^2＋）,1 UTaqDNA聚合酶,0.20 mmol/L dNTP,0.25μmol/L引物,DNA模板70-80 ng/μl,退火温度53-54℃。 |
| Author | 潘丽梅 朱建华 秦献泉 彭宏祥 卢美英 |
| AuthorAffiliation | 广西大学农学院,广西南宁530005 广西农业科学院园艺研究所,广西南宁530007 |
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| Author_FL | PAN Li-mei LU Mei-ying ZHU Jian-hua QIN Xian-quan PENG Hong-xiang |
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| Publisher | 广西大学农学院,广西,南宁530005%广西农业科学院园艺研究所,广西,南宁530007 |
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| Snippet | 应用改良的CTAB法提取龙荔基因组DNA,探讨龙荔ISSR-PCR扩增反应体系的建立和优化。结果表明,经过改良的CTAB法提取龙荔基因组DNA效果良好,所提取的DNA纯度较高,符合ISSR-PCR反应的要求;龙荔ISSR-PCR最佳反应体系（反应总体积20μl）：1×Buffer缓冲液（含适量Mg^2＋）,1... S667.1; 应用改良的CTAB法提取龙荔基因组DNA,探讨龙荔ISSR-PCR扩增反应体系的建立和优化.结果表明,经过改良的CTAB法提取龙荔基因组DNA效果良好,所提取的DNA纯度较高,符合ISSR-PCR反应的要求;龙荔ISSR-PCR最佳反应体系(反应总体积20 μl):1 ×... |
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| StartPage | 145 |
| SubjectTerms | DNA提取 ISSR-PCR 分子标记 龙荔 |
| Title | 龙荔基因组DNA的提取及ISSR-PCR体系的建立与优化 |
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