纤维素降解细菌DBJ的筛选鉴定及其特性研究

在纤维素刚果红培养基中筛选具有透明圈的方法,从肥沃的土壤中筛选获得一株溶解圈直径与菌落直径比值（D/d）可达4.75的细菌,命名为DBJ。该菌最适产纤维素酶的培养时间是60 h,培养温度是35℃,起始p H 7.0。通过形态学、生理生化试验和16S r DNA序列比对分析对DBJ进行了鉴定。结果表明,革兰氏阳性菌DBJ为芬氏纤维微菌（Cellulosimicrobium funkei）,将其命名为Cellulosimicrobium funkei DBJ,DBJ的16S r DNA序列的Gen Bank登录号为JX392404。...

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Bibliographic Details
Published in西南农业学报 Vol. 29; no. 1; pp. 81 - 84
Main Author 吴翔 甘炳成 黄忠乾 谭伟
Format Journal Article
LanguageChinese
Published 四川省农业科学院土壤肥料研究所,四川成都,610066 2016
Subjects
筛选
芬氏纤维微菌
鉴定
降解纤维素
降解纤维素
筛选
Cellulose-decomposing
鉴定
Isolation
Identification
芬氏纤维微菌
Cellulosimicrobium funkei
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Summary:在纤维素刚果红培养基中筛选具有透明圈的方法,从肥沃的土壤中筛选获得一株溶解圈直径与菌落直径比值（D/d）可达4.75的细菌,命名为DBJ。该菌最适产纤维素酶的培养时间是60 h,培养温度是35℃,起始p H 7.0。通过形态学、生理生化试验和16S r DNA序列比对分析对DBJ进行了鉴定。结果表明,革兰氏阳性菌DBJ为芬氏纤维微菌（Cellulosimicrobium funkei）,将其命名为Cellulosimicrobium funkei DBJ,DBJ的16S r DNA序列的Gen Bank登录号为JX392404。
Bibliography:WU Xiang;GAN Bing-cheng;HUANG Zhong-qian;TAN Wei （Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Sichuan Chengdu 610066, China）
51-1213/S
Cellulose-decomposing; Isolation; Identification; Cellulosimicrobium funkei
Based on the ratio of lysed zone diameter and colony diameter on the cellulose-congo red medium,a strain was isolated,its ratio was 4. 75,and designated it as DBJ. The optimum time,temperature and p H for DBJ decompose cellulose were 60 h,35 ℃ and 7. 0.Based on the morphological,physiological and phylogenetic analysis of 16 S r DNA,DBJ was identified. The result showed that DBJ was gram-positive bacterium. And DBJ was identified as Cellulosimicrobium funkei,and named Cellulosimicrobium funkei DBJ. The sequences of16 S r DNA of DBJ had been submitted to Gen Bank and the accession number was JX392404.
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2016.01.016