HPLC法测定冬柏通淋合剂中盐酸小檗碱的含量

[目的建立HPLC法测定冬柏通淋合剂中盐酸小檗碱的含量.方法采用KromasilC18色谱柱（150mm×4.6mm,5μm）;流动相：乙腈-0.05mol/L磷酸二氢钠佣磷酸调节pH=3）（28：72）;流速：1.0ml/min;柱温：室温（25℃）;检测波长：345nm.结果盐酸小檗碱在1.00-50.00μg/ml范围内线性关系良好（r=0.9996）,平均回收率为104.70%,RSD为1.60%（n=5）.结论该方法准确、灵敏度高、专属性强、重现性好,对提高冬柏通淋合剂的质量控制标准具有参考意义....

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Published in药学实践杂志 Vol. 35; no. 4; pp. 353 - 354
Main Author 侯晓丽 谭朝丹 时扣荣 顾伟鹰 陈伟成
Format Journal Article
LanguageChinese
Published 上海中医药大学附属第七人民医院,上海,200137 2017
Subjects
冬柏通淋合剂
盐酸小檗碱
高效液相色谱
高效液相色谱
冬柏通淋合剂
Dongbai Tonglin Heji
berberine hydrochloride
盐酸小檗碱
HPLC
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Summary:[目的建立HPLC法测定冬柏通淋合剂中盐酸小檗碱的含量.方法采用KromasilC18色谱柱（150mm×4.6mm,5μm）;流动相：乙腈-0.05mol/L磷酸二氢钠佣磷酸调节pH=3）（28：72）;流速：1.0ml/min;柱温：室温（25℃）;检测波长：345nm.结果盐酸小檗碱在1.00-50.00μg/ml范围内线性关系良好（r=0.9996）,平均回收率为104.70%,RSD为1.60%（n=5）.结论该方法准确、灵敏度高、专属性强、重现性好,对提高冬柏通淋合剂的质量控制标准具有参考意义.
Bibliography:31-1685/R
Objective To establish a HPLC method for determination of berberine hydrochloride in Dongbai Tonglin He-ji .Methods The separation column of Kromasil C18 （150 mm ×4 .6 mm ,5 μm ） was used . The mobile phase was acetonitrile and 0 .05 mol/L NaH2 PO4 solution （pH was adjusted to about 3 with phosphoric acid ） . The flow rate was 1 .0 ml/min with the column at room temperature and the detection wavelengths at 345 nm for berberine hydrochloride . Results The linear range of berberine hydrochloride was 1.00 - 50.00 pg/ml （ 0 .999 6） . The average recoveries of berberine hydrochloride were104 .70% （RSD 1 .60% , n= 5） . Conclusion This method is accurate , sensitive , selective and reproducible . It provides an al-ternative method to improve the quality control of Dongbai Tonglin Heji .
Dongbai Tonglin Heji ; berberine hydrochloride ; HPLC
HOU Xiaoli , TAN Zhaodan , SHI Kourong , GU Weiying , CHEN Weicheng （Department of Pharmacy , Seventh Peoples Hospi-tal Affiliated to Shanghai University of TCM , 200137 ,
ISSN:1006-0111
DOI:10.3969/j.issn.1006-0111.2017.04.016