Isolation and characterization of a novel analyte from Bacillus subtilis SC-8 antagonistic to Bacillus cereus

• Published in: Journal of bioscience and bioengineering Vol. 110; no. 3; pp. 298 - 303
• Main Authors: Lee, Nam Keun, Yeo, In-Cheol, Park, Joung Whan, Kang, Byung-Sun, Hahm, Young Tae
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.09.2010; Osaka, Japan: Society for Bioscience and Bioengineering, Japan; Amsterdam, the Netherlands: Distributed outside Japan by Elsevier Science; Elsevier
• Subjects: Antagonistic substance; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - metabolism; Anti-Bacterial Agents - pharmacology; Antibiotic-like substance; Apoptosis - drug effects; Bacillus; Bacillus cereus; Bacillus cereus - chemistry; Bacillus cereus - cytology; Bacillus cereus - drug effects; Bacillus licheniformis; Bacillus subtilis; Bacillus subtilis - classification; Bacillus subtilis - metabolism; Biological and medical sciences; Biotechnology; Foodborne pathogenic bacteria; Fundamental and applied biological sciences. Psychology; Glycine max - microbiology; Listeria monocytogenes; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Salmonella enterica; Salmonella enteritidis; Soybean-fermenting bacteria; Species Specificity; Staphylococcus aureus; Soybean-fermenting bacteria; Antagonistic substance; Bacillus cereus; Bacillus subtilis; Antibiotic-like substance; Foodborne pathogenic bacteria; Soybean; Bacillaceae; Fermentation; Characterization; Antibiotic; Bacillales; Pathogenic; Bacteria; Isolation; Antagonist
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2010.03.002

In this study, an effective substance was isolated from Bacillus subtilis SC-8, which was obtained from traditionally fermented soybean paste, cheonggukjang. The substance was purified by HPLC, and its properties were analyzed. It had an adequate antagonistic effect on Bacillus cereus, and its spectrum of activity was narrow. When tested on several gram-negative and gram-positive foodborne pathogenic bacteria such as Salmonella enterica, Salmonella enteritidis, Staphylococcus aureus, and Listeria monocytogenes, no antagonistic effect was observed. Applying the derivative from B. subtilis SC-8 within the same genus did not inhibit the growth of major soybean-fermenting bacteria such as Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloquefaciens. The range of pH stability of the purified antagonistic substance was wide (from 4.0 to >10.0), and the substance was thermally stable up to 60 °C. In the various enzyme treatments, the antagonistic activity of the purified substance was reduced with proteinase K, protease, and lipase; its activity was partially destroyed with esterase. Spores of B. cereus did not grow at all in the presence of 5 μg/mL of the purified antagonistic substance. The isolated antagonistic substance was thought to be an antibiotic-like lipopeptidal compound and was tentatively named BSAP-254 because it absorbed to UV radiation at 254 nm.