Intracellular Reactive Oxygen Species Production by Polymorphonuclear Leukocytes in Bovine Leukemia Virus-Infected Dairy Cows

• Published in: Journal of Veterinary Medical Science Vol. 74; no. 2; pp. 221 - 225
• Main Authors: SOUZA, Fernando Nogueira de, BLAGITZ, Maiara Garcia, LATORRE, Andréia Oliveira, SANCHEZ, Eduardo Milton RAMOS, BATISTA, Camila Freitas, WEIGEL, Rebeca Alves, RENNO, Francisco Palma, SUCUPIRA, Maria Claudia Araripe, Alice Maria Melville Paiva DELLA LIBERA
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2012; Japan Science and Technology Agency
• Subjects: Animals; Bovine leukemia virus; Cattle; deltaretrovirus; Enzootic Bovine Leukosis - blood; Enzootic Bovine Leukosis - immunology; Escherichia coli; Female; Flow Cytometry - veterinary; Leukemia Virus, Bovine - immunology; neutrophils; Neutrophils - metabolism; oxidative burst; Random Allocation; Reactive Oxygen Species - metabolism; Respiratory Burst - immunology; Staphylococcus aureus; Statistics, Nonparametric
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.11-0246

The present study assesses the oxidative burst activity from polymorphonuclear leukocytes (PMNLs) from bovine leukemia virus (BLV)-infected cows. Fifteen clinically healthy cows were divided into serologically positive cows without any hematological alteration, serologically positive animals with persistent lymphocytosis (PL) and healthy serologically negative cows. The oxidative burst activity from the PMNLs was evaluated by flow cytometry using 2',7'-dichlorofluorescein diacetate as a probe. PMNLs from each cow were incubated with heat-killed Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) to stimulate oxidative burst activity. The results of the present work showed no significant difference in the oxidative burst activity without any stimulus and elicited by S. aureus. Conversely, a decrease in the oxidative burst index induced by E. coli in PMNLs was observed in BLV-infected cows.