Role of corticotropin-releasing factor on bladder function in rats with psychological stress

• Published in: Scientific reports Vol. 9; no. 1; pp. 9828 - 7
• Main Authors: Seki, Masaya, Zha, Xin-Min, Inamura, So, Taga, Minekatsu, Matsuta, Yosuke, Aoki, Yoshitaka, Ito, Hideaki, Yokoyama, Osamu
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08.07.2019; Nature Publishing Group
• Subjects: 631/443/376; 631/45/776/1178; 692/4025/1334; 82/80; Acetylcholine receptors (muscarinic); Animals; Antalarmin; Bladder; Case-Control Studies; Corticotropin-releasing hormone; Corticotropin-Releasing Hormone - blood; Corticotropin-Releasing Hormone - metabolism; Disease Models, Animal; Humanities and Social Sciences; Injections, Intraperitoneal; Male; mRNA; multidisciplinary; Peptides; Pyrimidines - administration & dosage; Pyrimidines - pharmacology; Pyrroles - administration & dosage; Pyrroles - pharmacology; Rats; Rats, Sprague-Dawley; Receptor, Muscarinic M2 - genetics; Receptor, Muscarinic M2 - metabolism; Receptor, Muscarinic M3 - genetics; Receptor, Muscarinic M3 - metabolism; Receptors, Corticotropin-Releasing Hormone - genetics; Receptors, Corticotropin-Releasing Hormone - metabolism; Rodents; Science; Science (multidisciplinary); Stress, Psychological - drug therapy; Stress, Psychological - metabolism; Stress, Psychological - physiopathology; Urinary Bladder - drug effects; Urinary Bladder - metabolism; Urinary Bladder - physiopathology; Urination; Urination - drug effects; Viscera
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-46267-9

Stress-related peptide corticotropin-releasing factor (CRF) and CRF-related peptides are distributed in the peripheral viscera such as the bladder. We investigated the contribution of psychological stress (PS) and CRF on bladder function. Male rats received sham stress (SS) or PS using a communication box method for 120 min every day for 7 days. One group of rats received the intraperitoneal CRF-R1 antagonist antalarmin for 7 days during stress exposure. Mean voided volume per micturition was significantly lower in PS rats compared to SS rats, which was antagonized by antalarmin treatment. Increases in plasma and bladder CRF, and mRNA expressions of bladder CRF, CRF-R1, and M2/3 muscarinic receptors, were found in PS rats. CRF did not influence bladder contraction in itself; however, stress increased the response of muscarinic contraction of bladder strips. These changes were antagonized by antalarmin treatment. In conclusion, PS reinforces M3 receptor-mediated contractions via CRF-R1, resulting in bladder storage dysfunction.