The effect of stromelysin-1 (MMP-3) on non-collagenous extracellular matrix proteins of demineralized dentin and the adhesive properties of restorative resins

• Published in: Biomaterials Vol. 29; no. 33; pp. 4367 - 4373
• Main Authors: Boukpessi, T, Menashi, S, Camoin, L, TenCate, J.M, Goldberg, M, Chaussain-Miller, C
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.11.2008; Elsevier
• Subjects: Adhesion; Adhesives - metabolism; Adolescent; Advanced Basic Science; Cellular Biology; Child; Dentin; Dentin - chemistry; Dentin - metabolism; Dentin - ultrastructure; Dentistry; Extracellular matrix proteins (ECM); Extracellular Matrix Proteins - genetics; Extracellular Matrix Proteins - metabolism; Humans; Integrin-Binding Sialoprotein; Life Sciences; Matrix Metalloproteinase 3 - analysis; Matrix Metalloproteinase 3 - genetics; Matrix Metalloproteinase 3 - metabolism; Molecular Weight; Osteopontin - analysis; Osteopontin - chemistry; Osteopontin - metabolism; Proteoglycans; Proteoglycans - chemistry; Proteoglycans - metabolism; Recombinant Proteins - metabolism; Resins, Synthetic - metabolism; Sialoglycoproteins - chemistry; Sialoglycoproteins - metabolism; Stromelysin-1 (MMP-3); Time Factors; Dentin; Proteoglycans; Extracellular matrix proteins (ECM); Stromelysin-1 (MMP-3); Adhesion
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2008.07.035

Abstract Dentin non-collagenous matrix components (NCPs) are structural proteins involved in the formation, the architecture and the mineralization of the extracellular matrix (ECM). We investigated here how recombinant metalloproteinase stromelysin-1, also termed MMP-3, initiates the release of ECM molecules from artificially demineralized human dentin. Analysis of the supernatants by Western blotting reveals that MMP-3 extracts PGs (decorin, biglycan), and also a series of phosphorylated proteins: dentin sialoprotein (DSP), osteopontin (OPN), bone sialoprotein (BSP) and MEPE, but neither dentin matrix protein-1 (DMP1), another member of the SIBLING family, nor osteocalcin (OC), a non-phosphorylated matrix molecule. After treatment of dentin surfaces by MMP-3, scanning electron microscope (SEM) examination of resin replica shows an increased penetration of the resin into the dentin tubules when compared to surfaces only treated by demineralizing solutions. This preclinical investigation suggests that MMP-3 may be used to improve the adhesive properties of restorative materials.