Long noncoding RNA NEAT1 promotes laryngeal squamous cell cancer through regulating miR-107/CDK6 pathway

• Published in: Journal of experimental & clinical cancer research Vol. 35; no. 1; p. 22
• Main Authors: Wang, Peng, Wu, Tianyi, Zhou, Han, Jin, Qianqian, He, Guoqing, Yu, Haoyang, Xuan, Lijia, Wang, Xin, Tian, Linli, Sun, Yanan, Liu, Ming, Qu, Lingmei
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 29.01.2016; BioMed Central
• Subjects: Animals; Antisense RNA; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - pathology; Cell Cycle; Cell Line, Tumor; Cyclin-Dependent Kinase 6 - genetics; Cyclin-Dependent Kinase 6 - metabolism; Development and progression; Gene expression; Gene Expression Regulation, Neoplastic; Genetic aspects; Humans; Laryngeal cancer; Laryngeal Neoplasms - genetics; Laryngeal Neoplasms - metabolism; Laryngeal Neoplasms - pathology; Mice; MicroRNAs - genetics; Neoplasm Transplantation; Physiological aspects; RNA, Long Noncoding - genetics; Signal Transduction; China
• ISSN: 1756-9966; 0392-9078; 1756-9966
• DOI: 10.1186/s13046-016-0297-z

Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) plays key role in the progression of some human cancers. However, the role of NEAT1 in human laryngeal squamous cell cancer (LSCC) is still unknown. We therefore investigated the expression and function of NEAT1 in LSCC. NEAT1 level in LSCC and adjacent non-neoplastic tissues were detected by qRT-PCR. NEAT1 was knockdown in LSCC cells and cell proliferation, apoptosis and cell cycle were examined. The growth of xenografts with NEAT1 knockdown LSCC cells was analyzed. NEAT1 level was significantly higher in LSCC than in corresponding adjacent non-neoplastic tissues, and patients with neck nodal metastasis or advanced clinical stage had higher NEAT1 expression. Moreover, siRNA mediated NEAT1 knockdown significantly inhibited the proliferation and induced apoptosis and cell cycle arrest at G1 phase in LSCC cells. The growth of LSCC xenografts was significantly suppressed by the injection of NEAT1 siRNA lentivirus. Furthermore, NEAT1 regulated CDK6 expression in LSCC cells which was mediated by miR-107. NEAT1 plays an oncogenic role in the tumorigenesis of LSCC and may serve as a potential target for therapeutic intervention.