The effect of chemically modified electrospun silica nanofiber on the mRNA and miRNA expression profile of neural stem cell differentiation

• Published in: Journal of biomedical materials research. Part A Vol. 104; no. 11; pp. 2730 - 2743
• Main Authors: Mercado, Augustus T., Yeh, Jui-Ming, Chin, Ting Yu, Chen, Wen Shuo, Chen-Yang, Yui Whei, Chen, Chung-Yung
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.11.2016; Wiley Subscription Services, Inc
• Subjects: Animals; Apoptosis; Axonogenesis; Biocompatibility; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Cell differentiation; Cell proliferation; Cell Proliferation - drug effects; Cells, Cultured; Differentiation; Differentiation (biology); Dynamics; Electrospinning; expression profile; Female; Gene expression; Genes; In vitro methods and tests; Long-term potentiation; microarray; Microenvironments; MicroRNAs - genetics; miRNA; mRNA; Nanofibers; Nanofibers - chemistry; Nanofibers - ultrastructure; Nanomaterials; Nanostructure; Neural stem cells; Neural Stem Cells - cytology; Neural Stem Cells - drug effects; Neural Stem Cells - metabolism; Neurogenesis; Neurogenesis - drug effects; NSC differentiation; Rats, Sprague-Dawley; Regenerative medicine; Ribonucleic acids; RNA, Messenger - genetics; Silica; silica nanofiber; Silicon dioxide; Silicon Dioxide - chemistry; Silicon Dioxide - pharmacology; Stem cells; Therapeutic applications; Tissue engineering; Transcriptome - drug effects; miRNA; microarray; mRNA; silica nanofiber; NSC differentiation; expression profile
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.35819

A detailed genomic and epigenomic analyses of neural stem cells (NSCs) differentiation in synthetic microenvironments is essential for the advancement of regenerative medicine and therapeutic treatment of diseases. This study identified the changes in mRNA and miRNA expression profile during NSC differentiation on an artificial matrix. NSCs were grown on a surface‐modified, electrospun tetraethyl‐orthosilicate nanofiber (designated as SNF‐AP) by providing a 3D‐environment for cell growth and differentiation. Differentially expressed mRNAs and miRNAs of NSC differentiated in this microenvironment were identified through microarray analysis. The genes and miRNA targets responsible for the differentiation fate of NSCs and neuron development process were determined using Ingenuity Pathway Analysis (IPA). SNF‐AP enhanced the expression of genes that activates the proliferation, development, and outgrowth of neurons, differentiation and generation of cells, neuritogenesis, outgrowth of neurites, microtubule dynamics, formation of cellular protrusions, and long‐term potentiation during NSC differentiation. On the other hand, PDL inhibited neuritogenesis, microtubule dynamics, and proliferation and differentiation of cells and activated the apoptosis function. Moreover, the nanomaterial promoted the expression of more let‐7 miRNAs, which have vital roles in NSC differentiation. Overall, SNF‐AP is biocompatible and applicable scaffold for NSC differentiation in the development of neural tissue engineering. These findings are useful in enhancing in vitro NSC differentiation potential for preclinical studies and future clinical applications. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2730–2743, 2016.