Immune Response of Mice Infected with Recombinant Vaccinia Viruses Carrying the HIV gag Gene
We examined mouse immune response to 4 kinds of recombinant vaccinia viruses carrying the HIV gag gene, including vac-gag/pol, which produces HIV-like particles with processed gag proteins; vac-gag, which also produces HIV-like particles but with unprocessed gag protein; and vac-gag-pol-fuse and vac...
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Published in | MICROBIOLOGY and IMMUNOLOGY Vol. 35; no. 10; pp. 849 - 861 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Tokyo
Blackwell Publishing Ltd
01.01.1991
Center For Academic Publications Japan Center for Academic Publications Japan |
Subjects | |
Online Access | Get full text |
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Summary: | We examined mouse immune response to 4 kinds of recombinant vaccinia viruses carrying the HIV gag gene, including vac-gag/pol, which produces HIV-like particles with processed gag proteins; vac-gag, which also produces HIV-like particles but with unprocessed gag protein; and vac-gag-pol-fuse and vac-es-gag/pol, neither of which produces such particles but releases reverse transcriptase and gag protein, respectively, from infected cells. Although infection of mice with recombinant vaccinia viruses induced production of the anti-p24 antibody in all mice, vac-gag/pol and vac-es-pol induced higher production than the other two recombinants. Increase in [3H]thymidine uptake by splenic lymphocytes following p24 antigen stimulation was most evident in mice infected with vac-gag/pol. Thus, the highest immune reaction, both humoral and cellular, was elicited by vac-gag/pol, indicating that among those tested, this recombinant vaccinia virus is the best candidate for a vaccine that induces anti-HIV gag immunity. |
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Bibliography: | ark:/67375/WNG-G0FZTRZ1-P Ministry of Education, Science and Culture of Japan ArticleID:MIM02025 istex:220BEF5895532176FEF0C8F1C2870A2C2F4B648B ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0385-5600 1348-0421 |
DOI: | 10.1111/j.1348-0421.1991.tb02025.x |