宿根矮化病菌侵染后甘蔗防御酶活性变化

[目的]研究接种甘蔗宿根矮化病（Ratoon stuning disease,RSD）菌后甘蔗防御酶活性的变化,为进一步探明甘蔗与RSD病菌的互作机制提供理论依据.[方法]以温汤脱毒后的甘蔗品种桂糖1 1号（GT11）为材料,设RSD菌液浸种为处理,ddH2O浸种为对照,测定甘蔗茎、叶不同时期超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）、谷胱甘肽还原酶（GR）等5种防御酶活性变化.[结果]在RSD病菌胁迫下,甘蔗茎和叶片中相关防御酶活性均有所变化,茎中除APX活性一直高于对照外,其他酶活性均表现为在90 d时低于对照,180d时高于对照;叶...

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Bibliographic Details
Published in南方农业学报 Vol. 45; no. 9; pp. 1551 - 1557
Main Author 谢晓娜 张小秋 梁永检 杨丽涛 李杨瑞
Format Journal Article
LanguageChinese
Published 广西农业科学院/中国农业科学院甘蔗研究中心/广西甘蔗生物技术与遗传改良重点实验室/广西甘蔗遗传改良重点实验室,南宁530007 2014
广西大学农学院/亚热带农业生物资源保护与利用国家重点实验室,南宁,530005%广西大学农学院/亚热带农业生物资源保护与利用国家重点实验室,南宁530005
Subjects
宿根矮化病
甘蔗
防御酶活性
宿根矮化病
甘蔗
ratoon stunting disease(RSD)
防御酶活性
defense enzyme activities
sugarcane
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ISSN2095-1191
DOI10.3969/j.issn.2095-1191.2014.9.1551

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Summary:[目的]研究接种甘蔗宿根矮化病（Ratoon stuning disease,RSD）菌后甘蔗防御酶活性的变化,为进一步探明甘蔗与RSD病菌的互作机制提供理论依据.[方法]以温汤脱毒后的甘蔗品种桂糖1 1号（GT11）为材料,设RSD菌液浸种为处理,ddH2O浸种为对照,测定甘蔗茎、叶不同时期超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）、谷胱甘肽还原酶（GR）等5种防御酶活性变化.[结果]在RSD病菌胁迫下,甘蔗茎和叶片中相关防御酶活性均有所变化,茎中除APX活性一直高于对照外,其他酶活性均表现为在90 d时低于对照,180d时高于对照;叶中除POD活性一直高于对照外,其他酶活性在90 d时高于对照,180 d时低于对照.[结论]在RSD病菌胁迫下,甘蔗茎和叶片中CAT、SOD、POD、APX及GR活性均有所提高,随着胁迫时间的延长在茎中出现逐渐高于对照的趋势,叶片则与此相反.
Bibliography:[Objective]The present study was carried out to assess the changes of defense-related enzyme activi- ties in sugarcane under ratoon stunting disease （RSD）, in order to provide references for illustrating the mechanism of interaction between sugarcane and the bacteria of RSD. [Method]Sugarcane variety of GTll was selected as the plant materials. Seedcanes were treated at 52 ℃ for 30 min for pathogen free as a control and inoculated with Leifsonia xyli subsp. Xyli（Lxx）as RSD-infected sugarcane. The healthy and RSD-infected sugarcane was confirmed by PCR and the change of defense-related enzyme activity in sugarcane was analyzed in both treatments. [Result]The activities of CAT, SOD, POD and GR in stem were lower in RSD-infected treatment than those in control on the 90th day after transplant, while they were higher in treatment on the 180th day after transplant as comparison with the control except the activity of APX which was higher in treatment than this in control during sugarcane growth period. In contrast
ISSN:2095-1191
DOI:10.3969/j.issn.2095-1191.2014.9.1551