Correlation between Interleukin-17 gene polymorphism and osteoarthritis susceptibility in Han Chinese population

• Published in: BMC medical genetics Vol. 20; no. 1; p. 20
• Main Authors: Bai, Yuming, Gao, Shijun, Liu, Ying, Jin, Shengli, Zhang, Haisen, Su, Ke
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 18.01.2019; BioMed Central; BMC
• Subjects: Age; Aged; Alleles; Asian Continental Ancestry Group - ethnology; Asian Continental Ancestry Group - genetics; Breast cancer; Case-Control Studies; Chemokines; China - ethnology; Cytokines; Deoxyribonucleic acid; Disease; Disease susceptibility; DNA; EDTA; Female; Gastric cancer; Gene polymorphism; Genes; Genetic aspects; Genetic Association Studies; Genetic polymorphisms; Genetic Predisposition to Disease; Genomes; Genotypes; Han (Chinese people); Haplotypes; Health aspects; Humans; IL-17A; IL-17F; Inflammation; Inflammatory bowel disease; Inflammatory diseases; Interleukin 17; Interleukin-17 - blood; Interleukin-17 - genetics; Interleukins; Knee; Male; Medical research; Middle Aged; Osteoarthritis; Osteoarthritis, Knee - blood; Osteoarthritis, Knee - ethnology; Osteoarthritis, Knee - genetics; Pathogenesis; Polymerase chain reaction; Polymorphism; Polymorphism, Single Nucleotide; Population; Population studies; Principal components analysis; Restriction fragment length polymorphism; Rheumatoid arthritis; Rheumatology; Risk factors; Sequence Analysis, DNA; Severity of Illness Index; Smoking; Studies; China; Inflammation; IL-17F; IL-17A; Osteoarthritis; Polymorphism
• ISSN: 1471-2350; 1471-2350
• DOI: 10.1186/s12881-018-0736-0

Interleukin-17 (IL-17), a pleiotropic cytokine, plays a significant role in the inflammatory diseases. By a pilot study with small population, IL-17 polymorphisms (IL-17A rs2275913 and IL-17F rs763780) showed a more potential risk factor in knee osteoarthritis (OA) in our recruited subjects. In the current study, the association between IL-17A rs2275913 and IL-17F rs763780and the risk of OA in a Chinese population is studied. The IL-17A rs2275913 and IL-17F rs763780 polymorphisms were determined in 594 knee OA cases and 576 healthy controls, using polymerase chain reaction-restriction fragment length polymorphism assay. The relationship between genotype distribution and disease risk, as well as OA severity was analyzed by Chi-square test and multivariate logistic regression. The experimental results indicated that the polymorphism in IL-17 gene rs2275913 site were related to knee OA risk after the adjustment of BMI, sex, age, smoking and drinking status (AA vs. GG: odds ratio (OR), 1.411; 95% confidence interval (CI), 1.021-1.950; P = 0.040; A allele vs. G allele: OR, 1.192; P = 0.037; 95% CI, 1.012-1.404;). Similarly, subjects who are bearing the rs763780 variant genotypes (TC and CC) and C allele also had a higher susceptibility to knee OA compared with those who are bearing the TT genotype (TC vs. TT, OR: 1.312; P = 0.039; 95% CI: 1.017-1.692; CC vs. TT, OR: 2.812, P = 0.006, 95% CI: 1.338-5.909; C allele Vs. T allele, OR:1.413, P = 0.002, 95% CI:1.141-1.751). In the meantime, one high-risk haplotypes, AC (OR was 7.22, P < 0.01) was found. Both two polymorphisms do not correlated with OA severity based on Kellgren-Lawrence (K&L) scales. Finally, serum IL-17 levels of knee OA patients were greatly higher than those of controls (P = 0.001). With the limited size sample, our study shows that IL-17 gene polymorphisms possibly related to the high-risk knee OA occurrence.