Enhanced thermal stability of lichenase from Bacillus subtilis 168 by SpyTag/SpyCatcher-mediated spontaneous cyclization

• Published in: Biotechnology for biofuels Vol. 9; no. 76; p. 79
• Main Authors: Wang, Jindan, Wang, Yilin, Wang, Xinzhe, Zhang, Dandan, Wu, Shuyu, Zhang, Guangya
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 31.03.2016; BioMed Central
• Subjects: Analysis; Bacillus subtilis; Chemical synthesis; Chromatography; Denaturation; Enzymes; Molecular weight; Physiological aspects; Proteins; Temperature; Lichenase; Cyclization; Non-chromatographic purification; Thermal stability; SpyTag/SpyCatcher

SpyTag is a peptide that can form an irreversible covalent linkage to its 12 kDa partner SpyCatcher via a spontaneous isopeptide bond. Herein, we fused SpyTag at the N-terminal of lichenase and SpyCatcher at C-terminal so that the termini of lichenase were locked together by the covalent interaction...