Management of enzyme diversity in high-performance cellulolytic cocktails

• Published in: Biotechnology for biofuels Vol. 10; no. 1; p. 156
• Main Authors: Reyes-Sosa, Francisco Manuel, López Morales, Macarena, Platero Gómez, Ana Isabel, Valbuena Crespo, Noelia, Sánchez Zamorano, Laura, Rocha-Martín, Javier, Molina-Heredia, Fernando P, Díez García, Bruno
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 19.06.2017; BioMed Central; BMC
• Subjects: Alternative energy sources; Ascomycota; Biodiesel fuels; Bioethanol; Biological diversity; Biomass; Biorefineries; Carbohydrates; Cellulase; Cellulolytic cocktail improvement; Cellulolytic fungi; Cellulolytic microorganisms; Cellulose; Clonal deletion; Efficiency; Environmental conditions; Enzymatic hydrolysis; Enzymes; Fungi; Hydrolysis; Laboratories; Lignocellulose; Lignocellulosic biomass; Management; Microorganisms; Proteins; Proteomics; Refining; Substrates; Sugar; Bioethanol; Cellulolytic cocktail improvement; Lignocellulosic biomass; Enzymatic hydrolysis
• ISSN: 1754-6834; 1754-6834
• DOI: 10.1186/s13068-017-0845-6

Modern biorefineries require enzymatic cocktails of improved efficiency to generate fermentable sugars from lignocellulosic biomass. Cellulolytic fungi, among other microorganisms, have demonstrated the highest potential in terms of enzymatic productivity, complexity and efficiency. On the other hand, under cellulolytic-inducing conditions, they often produce a considerable diversity of carbohydrate-active enzymes which allow them to adapt to changing environmental conditions. However, industrial conditions are fixed and adjusted to the optimum of the whole cocktail, resulting in underperformance of individual enzymes. One of these cellulolytic cocktails from has been analyzed here by means of LC-MS/MS. Pure GH6 family members detected have been characterized, confirming previous studies, and added to whole cocktails to compare their contribution in the hydrolysis of industrial substrates. Finally, independent deletions of two GH6 family members, as an example of the enzymatic diversity management, led to the development of a strain producing a more efficient cellulolytic cocktail. These data indicate that the deletion of noncontributive cellulases (here EG VI) can increase the cellulolytic efficiency of the cocktail, validating the management of cellulase diversity as a strategy to obtain improved fungal cellulolytic cocktails.