Co-administration of berberine and plant stanols synergistically reduces plasma cholesterol in rats

• Published in: Atherosclerosis Vol. 201; no. 1; pp. 101 - 107
• Main Authors: Jia, Xiaoming, Chen, Yanfeng, Zidichouski, Jeffrey, Zhang, Junzeng, Sun, Changhao, Wang, Yanwen
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ireland Ltd 01.11.2008; Elsevier
• Subjects: Administration, Oral; Animals; Anticholesteremic Agents - administration & dosage; Atherosclerosis (general aspects, experimental research); Berberine; Berberine - administration & dosage; Biological and medical sciences; Blood and lymphatic vessels; Cardiology. Vascular system; Cardiovascular; Cholesterol - blood; Coronary heart disease; Diet, Atherogenic; Drug Therapy, Combination; Heart; Lipoproteins - blood; Liver cholesterol; Male; Medical sciences; Phytosterols - administration & dosage; Plant stanols; Plasma cholesterol; Plasma triacylglyceride; Rats; Rats, Sprague-Dawley; Sitosterols - administration & dosage; Toxicity; Triglycerides - blood; triacylglycerides; PS; LDL-C; Toxicity; HDL-cholesterol; T-C; acetyl-CoA carboxylase; AMP-activated protein kinase; berberine chloride; total cholesterol; Plasma triacylglyceride; body weight; HDL-C; LDL-cholesterol; BBR; BBRPS; BW; non-HDL-C; Liver cholesterol; ACC; Berberine; Plasma cholesterol; non-HDL-cholesterol (VLDL-cholesterol + intermediate density lipoprotein cholesterol + LDL-cholesterol); Rats; combination of BBR and plant stanols; control; CT; AMPK; TAG; plant stanols; Plant stanols; Rat; Digestive system; Liver; Rodentia; Lipids; Cardiovascular disease; Cholesterol; Vascular disease; Vertebrata; Mammalia; Animal; Atherosclerosis
• ISSN: 0021-9150; 1879-1484
• DOI: 10.1016/j.atherosclerosis.2008.03.008

Abstract The objective of the present study was to determine the beneficial effects and the safety of oral administration of the combination of berberine (BBR) and plant stanols (PS) on plasma lipid profiles in male Sprague–Dawley rats. Four groups of animals were fed a cornstarch–casein–sucrose-based high-cholesterol (2%, w:w) and high-fat (27.5%) diet. Three treatment groups were supplemented with either BBR (100 mg kg−1 body weight d−1 ), PS (1% in diet, w:w), or the combination of both (BBRPS). After 6 wk, animals were sacrificed and followed immediately with the collection of blood and organ samples. Lipid analysis revealed that PS lowered plasma total cholesterol (T-C) by 18% ( p = 0.067) and non-HDL-cholesterol (non-HDL-C) by 29% ( p = 0.013) as compared with the control, while BBR had no effect on both T-C and non-HDL-C. The combination treatment of BBRPS reduced plasma T-C by 41% ( p = 0.0002) and non-HDL-C by 59% ( p < 0.0001) compared to the control group. BBR reduced plasma TG levels by 31% at a marginal significance relative to the control ( p = 0.054), whereas PS had no effect. BBRPS showed an additive effect of BBR and PS on plasma TAG. PS and BBRPS both decreased liver cholesterol ( p = 0.0027 and 0.0002, respectively). BBR and PS, either alone or in combination, did not show any toxic effects as assessed by plasma concentration of hepatic biochemical parameters. These results demonstrate that BBR and PS, when combined, synergistically lower plasma cholesterol levels and significantly reduce liver cholesterol, without the observation of any toxic effects.