Construction, Phenotypic Analysis, and Immunogenicity of a UL5/UL29 Double Deletion Mutant of Herpes Simplex Virus 2

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Published inJournal of Virology Vol. 74; no. 17; pp. 7963 - 7971
Main Authors Da Costa, X, Kramer, M F, Zhu, J, Brockman, M A, Knipe, D M
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.09.2000
Subjects
Animals
Blotting, Southern
Blotting, Western
Cell Line
Cercopithecus aethiops
dl5-29 deletion
Enzyme-Linked Immunosorbent Assay
Female
Gene Deletion
herpes simplex virus 2
Herpesvirus 2, Human - genetics
Herpesvirus 2, Human - metabolism
Herpesvirus 2, Human - physiology
Humans
Mice
Mice, Inbred BALB C
Mutagenesis, Site-Directed
Phenotype
Sequence Analysis, DNA
UL29 gene
UL5 gene
Vaccines and Antiviral Agents
Vero Cells
Viral Plaque Assay
Viral Proteins - analysis
Viral Proteins - biosynthesis
Viral Proteins - genetics
Viral Proteins - immunology
Viral Vaccines - biosynthesis
Viral Vaccines - genetics
Viral Vaccines - immunology
Virus Replication
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content type line 23
Present address: Wyeth Lederle Vaccines, Pearl River, NY 10965.
Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics and Committee on Virology, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115. Phone: (617) 432-1934. Fax: (617) 432-0223. E-mail address: david_knipe@hms.harvard.edu.
ISSN:0022-538X
1098-5514
DOI:10.1128/JVI.74.17.7963-7971.2000