A new nanoscale transdermal drug delivery system: oil body-linked oleosin-hEGF improves skin regeneration to accelerate wound healing

• Published in: Journal of nanobiotechnology Vol. 16; no. 1; p. 62
• Main Authors: Qiang, Weidong, Zhou, Tingting, Lan, Xinxin, Zhang, Xiaomei, Guo, Yongxin, Noman, Muhammad, Du, Linna, Zheng, Jie, Li, Wenqing, Li, Haoyang, Lu, Yubin, Wang, Hongyu, Guan, Lili, Zhang, Linbo, Li, Xiaokun, Yang, Jing, Li, Haiyan
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 30.08.2018; BioMed Central; BMC
• Subjects: Absorption; Carthamus tinctorius; Cell proliferation; Collagen; Drug delivery; Drug delivery systems; Epidermal growth factor; Epidermal growth factors; Fibroblast growth factor 2; Genetic aspects; Growth factors; Health aspects; Immunohistochemistry; Kinases; Medical research; Neonates; Oil; Oil body; Oleosin; Particle size; Proteins; Purification; Rats; Regeneration; Regeneration (Biology); Safflower oil; Seeds; Skin; Skin regeneration; Testing; Transdermal drug delivery systems; Transdermal medication; Transforming growth factor-b1; Vascular endothelial growth factor; Wound healing; Immunohistochemistry; Epidermal growth factor; Wound healing; Skin regeneration; Oil body
• ISSN: 1477-3155; 1477-3155
• DOI: 10.1186/s12951-018-0387-5

Epidermal growth factor (EGF) can promote cell proliferation as well as migration, which is feasible in tissue wound healing. Oil bodies have been exploited as an important platform to produce exogenous proteins. The exogenous proteins were expressed in oil bodies from plant seeds. The process can reduce purification steps, thereby significantly reducing the purification cost. Mostly, the diameter of oil body particle ranges between 1.0 and 1.5 µm in the safflower seeds, however, it reduces to 700-1000 nm in the transgenic safflower seeds. The significant reduction of particle size in transgenic seeds is extremely beneficial to skin absorption. The diameter of oil body in the transgenic safflower seeds was recorded in the range of 700-1000 nm. The smaller particle size improved their skin absorption. The expression level of oleosin-hEGF-hEGF in T3 transgenic seeds was highest at 69.32 mg/g of seeds. The oil body expressing oleosin-hEGF-hEGF had significant proliferative activity on NIH/3T3 cells and improved skin regeneration thereby accelerating wound healing in rats. The wound coverage rate exceeded 98% after treatment for 14 days with oil body expressing oleosin-hEGF-hEGF, while the saline without EGF group and wild type oil body group both showed less than 80%. The neonatal fibroblast and collagen were found to be increased in the safflower oil body expressing oleosin-hEGF-hEGF treatment group. TGF-β1, bFGF and VEGF were noted as important growth factors in the repair of cutaneous wounds. Their expression level increased after 4 and 7 day treatment, but decreased after 14 days. Therefore, it can promote skin regeneration to accelerate wounds healing. The expression of oleosin-hEGF-hEGF in T3 transgenic seeds was 80.43 ng/μL oil body. It had significant proliferative activity on NIH/3T3 cells and improved skin regeneration to accelerate wound healing in rats. The expression process of TGF-β1, bFGF and VEGF increased at first and then gradually declined.