Identification of candidate chemosensory genes in Mythimna separata by transcriptomic analysis

• Published in: BMC genomics Vol. 19; no. 1; pp. 518 - 14
• Main Authors: Du, Lixiao, Zhao, Xincheng, Liang, Xiangzhi, Gao, Xiwu, Liu, Yang, Wang, Guirong
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 04.07.2018; BioMed Central; BMC
• Subjects: Agrochemicals; Analysis; Animals; Antennae; Army-worms; Arthropod Antennae - metabolism; Bioinformatics; Cereal crops; Chemical senses; Chemoreception; Chemosensory gene; Diptera - genetics; DNA sequencing; Drosophila melanogaster; Economic analysis; Economic importance; Expression analysis; Female; Gene expression; Gene Expression Profiling; Gene sequencing; Genes; Genetic aspects; Genomes; Genomics; Helicoverpa virescens; Identification; Insect Proteins - classification; Insect Proteins - genetics; Insects; Ion channels (ligand-gated); Labial palps; Lepidoptera; Male; Membrane proteins; Membrane Proteins - classification; Membrane Proteins - genetics; Mythimna separata; Nerve Tissue Proteins - classification; Nerve Tissue Proteins - genetics; Neurons; Nucleotide sequencing; Odorant receptors; Odorant-binding protein; Oviposition; Pests; Pheromones; Phylogeny; Polymerase chain reaction; Proteins; Proteomics; Receptors; Receptors, Ionotropic Glutamate - classification; Receptors, Ionotropic Glutamate - genetics; Receptors, Odorant - classification; Receptors, Odorant - genetics; Reproductive strategy; RNA - chemistry; RNA - isolation & purification; RNA - metabolism; Sequence Analysis, RNA; Signal transduction; Site selection; Taste receptors; Transcriptome; Expression analysis; Chemosensory gene; Mythimna separata; Transcriptome
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-018-4898-0

The oriental armyworm, Mythimna separata, is an economically important and common Lepidopteran pest of cereal crops. Chemoreception plays a key role in insect life, such as foraging, oviposition site selection, and mating partners. To better understand the chemosensory mechanisms in M. separata, transcriptomic analysis of antennae, labial palps, and proboscises were conducted using next-generation sequencing technology to identify members of the major chemosensory related genes. In this study, 62 putative odorant receptors (OR), 20 ionotropic receptors (IR), 16 gustatory receptors (GR), 38 odorant binding proteins (OBP), 26 chemosensory proteins (CSP), and 2 sensory neuron membrane proteins (SNMP) were identified in M. separata by bioinformatics analysis. Phylogenetic analysis of these candidate proteins was performed. Differentially expressed genes (DEGs) analysis was used to determine the expressions of all candidate chemosensory genes and then the expression profiles of the three families of receptor genes were confirmed by real-time quantitative RT-PCR (qPCR). The important genes for chemoreception have now been identified in M. separata. This study will provide valuable information for further functional studies of chemoreception mechanisms in this important agricultural pest.