Development of diagnostic molecular markers for marker-assisted breeding against bacterial wilt in tomato

Bacterial wilt, caused by the Ralstonia pseudosolanacearum species complex, is an important vascular disease that limits tomato production in tropical and subtropical regions. Two major quantitative trait loci (QTL) of bacterial wilt resistance on chromosome 6 (Bwr-6) and 12 (Bwr-12) were previously...

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Published inBreeding Science Vol. 70; no. 4; pp. 462 - 473
Main Authors Abebe, Alebel Mekuriaw, Choi, Jinwoo, Kim, Youngjun, Oh, Chang-Sik, Yeam, Inhwa, Nou, Ill-Sup, Lee, Je Min
Format Journal Article
LanguageEnglish
Published Tokyo Japanese Society of Breeding 01.01.2020
Japan Science and Technology Agency
Subjects
Bacteria
bacterial wilt
Chromosome 12
Chromosome 6
Chromosomes
Cultivars
Diagnostic systems
Gene mapping
marker-assisted breeding
Markers
molecular marker
Plant breeding
polygenic resistance
Population genetics
Quantitative trait loci
Ralstonia pseudosolanacearum
Research Paper
single nucleotide polymorphism
Solanum lycopersicum
tomato
Tomatoes
Wilt
Hawaii
United States > US
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Summary:Bacterial wilt, caused by the Ralstonia pseudosolanacearum species complex, is an important vascular disease that limits tomato production in tropical and subtropical regions. Two major quantitative trait loci (QTL) of bacterial wilt resistance on chromosome 6 (Bwr-6) and 12 (Bwr-12) were previously identified in Solanum lycopersicum ‘Hawaii 7996’; however, marker-assisted breeding for bacterial wilt resistance is not well established. To dissect the QTL, six cleaved amplified polymorphic sites (CAPS) and derived CAPS (dCAPS) markers within the Bwr-6 region and one dCAPS marker near Bwr-12 were developed, and resistance levels in 117 tomato cultivars were evaluated. Two markers, RsR6-5 on chromosome 6 and RsR12-1 on chromosome 12, were selected based on the genotypic and phenotypic analysis. The combination of RsR6-5 and RsR12-1 effectively distinguishes resistant and susceptible cultivars. Furthermore, the efficiency of the two markers was validated in the F3 generation derived from the F2 population between E6203 (susceptible) and Hawaii 7998 (resistant). Resistant alleles at both loci led to the resistance to bacterial wilt. These markers will facilitate marker-assisted breeding of tomato resistant to bacterial wilt.
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These authors contributed equally to this work
Communicated by Sang-Nag Ahn
ISSN:1344-7610
1347-3735
DOI:10.1270/jsbbs.20027