The effect of tadalafil therapy on kidney damage caused by sepsis in a polymicrobial septic model induced in rats: a biochemical and histopathological study

• Published in: International Brazilian Journal of Urology Vol. 43; no. 2; pp. 345 - 355
• Main Authors: Benli, Erdal, Ayyildiz, Sema Nur, Cirrik, Selma, Koktürk, Sibel, Cirakoglu, Abdullah, Noyan, Tevfik, Ayyildiz, Ali, Germiyanoglu, Cankon
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira de Urologia 01.04.2017
• Subjects: Acute Kidney Injury; Animals; Calcitonin - blood; Catalase - analysis; Creatinine - blood; Cystatin C - blood; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; Interleukin-6 - blood; Kidney - drug effects; Kidney - pathology; Kidney Failure, Chronic; Ligation; Male; Malondialdehyde - analysis; Original; Peroxidase - analysis; Phosphodiesterase 5 Inhibitors - therapeutic use; Random Allocation; Rats, Wistar; Reference Values; Renal Insufficiency; Renal Insufficiency - etiology; Renal Insufficiency - pathology; Renal Insufficiency - prevention & control; Reproducibility of Results; Sepsis - complications; Sepsis - pathology; Sepsis - prevention & control; Spectrophotometry; Superoxide Dismutase - analysis; Tadalafil; Tadalafil - therapeutic use; Tadalafil; Acute Kidney Injury; Renal Insufficiency
• ISSN: 1677-5538; 1677-6119; 1677-6119; 1677-5538
• DOI: 10.1590/s1677-5538.ibju.2016.0075

Sepsis is an inflammatory reaction to bacteria involving the whole body and is a significant cause of mortality and economic costs. The purpose of this research was to determine whether tadalafil exhibits a preventive effect on sepsis in a septic model induced in rats with cecal ligation and puncture (CLP). Rats were randomly separated into groups, 10 rats in each: (i) a sham (control) group, (ii) an untreated sepsis group, (iii) a sepsis group treated with 5mg/kg tadalafil and (iv) a sepsis group treated with 10mg/kg tadalafil. A polymicrobial sepsis model was induced in rats using CLP. Rats were sacrificed after 16h, and blood and kidney tissues were collected for biochemical and histopathological study. Levels of the inflammatory parameter IL-6 decreased significantly in the sepsis groups receiving tadalafil in comparison with the untreated sepsis group (p < 0.05). In terms of histopathology, inflammation scores investigated in kidney tissues decreased significantly in the sepsis groups receiving tadalafil compared to the untreated sepsis group (p < 0.05). In addition, levels of creatinine and cystatin C measured in septic rats receiving tadalafil were lower by a clear degree than in septic rats (p < 0.05). In this study, tadalafil exhibited a preventive effect for sepsis-related damage by suppressing inflammation in serum and kidney tissue of septic rats in a polymicrobial sepsis model induced with CLP.