LncRNA-MSC-AS1 inhibits the ovarian cancer progression by targeting miR-425-5p

• Published in: Journal of ovarian research Vol. 14; no. 1; p. 109
• Main Authors: Zhao, Yinling, Yuan, Donglan, Zhu, Dandan, Xu, Tianhui, Huang, Aihua, Jiang, Li, Liu, Chiwen, Qian, Hua, Bu, Xinhua
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 28.08.2021; BioMed Central; BMC
• Subjects: Analysis; Apoptosis; Apoptosis - physiology; Breast cancer; Cell adhesion & migration; Cell cycle; Cell growth; Cell proliferation; Cell Proliferation - physiology; Colorectal cancer; Development and progression; Disease Progression; Female; Flow cytometry; Health aspects; Humans; Liver cancer; LncRNA MSC-AS1; Medical diagnosis; Medical prognosis; Medical research; Metastasis; MicroRNA; MicroRNA-425-5p; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Non-coding RNA; Ovarian cancer; Ovarian Neoplasms - genetics; Ovarian Neoplasms - metabolism; Ovarian Neoplasms - pathology; Polymerase chain reaction; Proliferation; Prostate cancer; Proteins; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Therapeutic targets; Tumor cell lines; China; United States > US; Japan; Proliferation; MicroRNA-425-5p; LncRNA MSC-AS1; Apoptosis; Ovarian cancer
• ISSN: 1757-2215; 1757-2215
• DOI: 10.1186/s13048-021-00857-2

Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) were reported to be aberrantly expressed and related to the pathogenesis of ovarian cancer. However, the role and regulatory mechanism of MSC-AS1 in ovarian cancer has yet to be fully elucidated. Expression of lncRNA MSC-AS1 (MSC-AS1) and microRNA-425-5p (miR-425-5p) in the ovarian cancer tissue samples and cell lines was examined by quantitative real-time polymerase chain reaction (qRT-PCR). The functions of MSC-AS1 on ovarian cancer cell proliferation, cell cycle and apoptosis were determined using MTT, colony formation and flow cytometry analyses. The protein expression levels were evaluated using western blot assay. The targeting relationship MSC-AS1 and miR-425-5p was verified via dual-luciferase reporter assay. MSC-AS1 expression level was lowly expressed, while miR-425-5p level was highly in ovarian cancer tissues and cells. Elevation of MSC-AS1 has the ability to significantly inhibit cell proliferation and facilitate cell apoptosis in SKOV3 and A2780 cells. Moreover, MSC-AS1 targeted and negatively modulated miR-425-5p. MiR-425-5p up-regulation has been proved to partially reverse the tumor suppressive function of MSC-AS1 overexpression CONCLUSION: MSC-AS1 sponged miR-425-5p to inhibit the ovarian cancer progression. These findings may provide a promising therapeutic target for the treatment of ovarian cancer.