The coupling of transcriptome and proteome adaptation during development and heat stress response of tomato pollen

• Published in: BMC genomics Vol. 19; no. 1; p. 447
• Main Authors: Keller, Mario, Simm, Stefan
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 08.06.2018; BioMed Central; BMC
• Subjects: Abiotic stress; Adaptation, Physiological - genetics; Analysis; Biodegradation; Bioinformatics; Coupling (molecular); Decoupling; Developmental stages; Gene expression; Gene Expression Profiling; Genetic aspects; Genomics; Heat; Heat shock proteins; Heat stress; Heat tolerance; Heat-Shock Response - genetics; High temperature; Homeostasis; Initiation factors; Meiosis; Metabolism; Molecular chains; Multivariate analysis; Pennisetum glaucum; Physiological aspects; Plant reproduction; Pollen; Pollen - genetics; Pollen - growth & development; Pollen - metabolism; Pollen - physiology; Pollen development; Pollen tube; Proteasomes; Proteins; Proteomes; Proteomics; Ribosomal proteins; Solanum lycopersicum - genetics; Solanum lycopersicum - growth & development; Solanum lycopersicum - metabolism; Solanum lycopersicum - physiology; Stress (Physiology); Stress response; Temperature effects; Tomatoes; Transcription; Transcription factors; Transcriptomics; Viability; Germany; Pollen development; Heat stress; Transcriptomics; Proteomics
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-018-4824-5

Pollen development is central for plant reproduction and is assisted by changes of the transcriptome and proteome. At the same time, pollen development and viability is largely sensitive to stress, particularly to elevated temperatures. The transcriptomic and proteomic changes during pollen development and of different stages in response to elevated temperature was targeted to define the underlying molecular principles. The analysis of the transcriptome and proteome of Solanum lycopersicum pollen at tetrad, post-meiotic and mature stage before and after heat stress yielded a decline of the transcriptome but an increase of the proteome size throughout pollen development. Comparison of the transcriptome and proteome led to the discovery of two modes defined as direct and delayed translation. Here, genes of distinct functional processes are under the control of direct and delayed translation. The response of pollen to elevated temperature occurs rather at proteome, but not as drastic at the transcriptome level. Heat shock proteins, proteasome subunits, ribosomal proteins and eukaryotic initiation factors are most affected. On the example of heat shock proteins we demonstrate a decoupling of transcript and protein levels as well as a distinct regulation between the developmental stages. The transcriptome and proteome of developing pollen undergo drastic changes in composition and quantity. Changes at the proteome level are a result of two modes assigned as direct and delayed translation. The response of pollen to elevated temperature is mainly regulated at the proteome level, whereby proteins related to synthesis and degradation of proteins are most responsive and might play a central role in the heat stress response of pollen.