Effects of factor v Leiden polymorphism on the pathogenesis and outcomes of preeclampsia

• Published in: BMC medical genetics Vol. 20; no. 1; pp. 189 - 6
• Main Authors: Ababio, G. K., Adu-Bonsaffoh, K., Abindau, E., Narh, G., Tetteh, D., Botchway, F., Morvey, D., Neequaye, J., Quaye, I. K.
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 27.11.2019; BioMed Central; BMC
• Subjects: Adult; Alleles; Automation; Births; Blood pressure; Blood tests; Coagulation factors; Data security; Dehydrogenases; Development and progression; Disease; Enzymes; Ethical aspects; Etiology; Etiology (Medicine); Exons; Factor V; Factor V - genetics; Female; Gene Frequency; Gene polymorphism; Genetic aspects; Genetic polymorphisms; Glycosylated hemoglobin; Health; Hemoglobin; Hemoglobins; Hepatocytes; Humans; Hypertension; Leiden; Leukocytes; Liver; Maternal mortality; Medical records; Mutation; Pathogenesis; Polymerase chain reaction; Polymorphism; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Pre-eclampsia; Pre-Eclampsia - genetics; Pre-Eclampsia - pathology; Preeclampsia; Pregnancy; Pregnancy Outcome; Proteins; Proteinuria; Purines; Restriction; Restriction fragment length polymorphism; Teaching hospitals; Uric acid; Urine; Variables; Womens health; Canada; Ghana; Factor V; Polymerase chain reaction; Restriction; Leiden; Preeclampsia
• ISSN: 1471-2350; 1471-2350
• DOI: 10.1186/s12881-019-0924-6

Factor V Leiden polymorphism is a well-recognized genetic factor in the etiology of preeclampsia. Considering that Ghana is recording high incidence of preeclampsia, we examined if factor V Leiden is a contributory factor to its development and pregnancy outcomes. STROBE consensus checklist was adopted to recruit eighty-one (81) consenting subjects after ethical clearance. Subjects were followed up till delivery to obtain outcomes of PE. Routine blood chemistry and proteinuria were done on all samples. Factor V Leiden was characterized by polymerase chain reaction and restriction fragment length polymorphism (RFLP). The data was captured as protected health information (PHI) and analyzed with SPSS version 22. Overall allelic frequencies found in FVL exon 10 were 0.67 and 0.33 for G and A alleles respectively. The FVL mutation was more in PE and hypertensive patients. Increased white blood cells, increased uric acid and a three - fold increment of AST / ALT ratio was observed in PE cases when stratified by FVL exons (exon 8 and 10). Significant differences were also observed between FVL and age, systolic blood pressure (SBP), diastolic blood pressure (DBP), liver enzymes, white blood cells (wbc), hemoglobin levels. FVL mutation allele frequency was 0.33, a first report. The mutation was associated with increased uric acid, liver enzymes and blood cell indices suggestive of acute inflammation.