Nanopore sequencing of cerebrospinal fluid of three patients with cryptococcal meningitis

• Published in: European journal of medical research Vol. 27; no. 1; p. 1
• Main Authors: Jin, Ke, Wang, Xiaojuan, Qin, Lingzhi, Jia, Yazhen, Zhou, Keke, Jiang, Yusu, Zhang, Milan, Zhang, Tao, Zhang, Mengge, Ma, Weifeng, Jia, Lin, Teng, Yongshi, Dai, Shuhua, Li, Wei
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 03.01.2022; BioMed Central; BMC
• Subjects: Acquired immune deficiency syndrome; AIDS; Case Report; Cerebrospinal fluid; Cryptococcal meningitis; DNA sequencing; Drug resistance; Health aspects; HIV; Human immunodeficiency virus; India; Magnetic resonance imaging; Mortality; Nanopore sequencing; Next-generation sequencing; Nucleotide sequencing; Printing-ink; India; Cryptococcal meningitis; Next-generation sequencing; Cerebrospinal fluid; Nanopore sequencing
• ISSN: 2047-783X; 0949-2321; 2047-783X
• DOI: 10.1186/s40001-021-00625-4

Cryptococcal meningitis (CM) has a high morbidity and mortality due to the low detection of Cryptococcus in cerebrospinal fluid (CSF) during the early stage of the disease with traditional methods. In addition to the traditional methods of India ink staining and cryptococcal antigen (CrAg), we used nanopore sequencing and next-generation sequencing (NGS) to detect pathogenic DNA in CSF samples of three patients with CM. The CSF samples of all three patients were positive by India ink staining and CrAg. NGS also detected Cryptococcus in all three CSF samples. Nanopore sequencing detected Cryptococcus in two CSF samples. Nanopore sequencing may be useful in assisting with the clinical diagnosis of CM. Further research is needed to determine the sensitivity and specificity of nanopore sequencing of CSF.