Quantitation of Major Human Cutaneous Bacterial and Fungal Populations

• Published in: Journal of Clinical Microbiology Vol. 48; no. 10; pp. 3575 - 3581
• Main Authors: Gao, Zhan, Perez-Perez, Guillermo I, Chen, Yu, Blaser, Martin J
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.10.2010; American Society for Microbiology (ASM)
• Subjects: Adult; Bacteria - isolation & purification; Bacterial Load; Bacteriology; Biological and medical sciences; Colony Count, Microbial; Corynebacterium; DNA, Fungal - genetics; DNA, Ribosomal - genetics; DNA, Ribosomal Spacer - genetics; Female; Fundamental and applied biological sciences. Psychology; Humans; Malassezia; Malassezia - isolation & purification; Male; Microbiology; Middle Aged; Miscellaneous; Polymerase Chain Reaction - methods; Propionibacterium; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Skin - microbiology; Staphylococcus; Streptococcus; Human; Bacteria; Quantitative analysis
• ISSN: 0095-1137; 1098-660X; 1098-660X
• DOI: 10.1128/JCM.00597-10

Because the human skin microbiota may play roles in the causation or modification of skin diseases, we sought to provide initial quantitative analysis from different cutaneous locations. We developed quantitative PCRs to enumerate the total bacterial and fungal populations, as well as the most common bacterial and fungal genera present in six locales, in eight healthy subjects. We used a set of primers and TaqMan MGB probes based on the bacterial 16S rRNA and fungal internally transcribed spacer region, as well as bacterial genus-specific probes for Propionibacterium, Corynebacterium, Streptococcus, and Staphylococcus and a fungal genus-specific probe for MALASSEZIA: The extent of human DNA contamination of the specimen was determined by quantitating the human housekeeping GAPDH gene. The highest level of 16S rRNA copies of bacteria was present in the axilla (4.44 ± 0.18 log₁₀ copies/μl [mean ± standard error of the mean]), with normalization based on GAPDH levels, but the other five locations were similar to one another (range, 2.48 to 2.89 log₁₀ copies/μl). There was strong symmetry between the left and right sides. The four bacterial genera accounted for 31% to 59% of total bacteria, with the highest percent composition in the axilla and the lowest in the forearm. Streptococcus was the most common genus present on the forehead and behind the ear. Corynebacterium spp. were predominant in the axilla. Fungal levels were 1 to 2 log₁₀ lower than for bacteria, with Malassezia spp. accounting for the majority of fungal gene copies. These results provide the first quantitation of the site and host specificities of major bacterial and fungal populations in human skin and present simple methods for their assessment in studies of disease.