Improvement of Fusing Ability of Human Sperm to Zona-Free Hamster Eggs by Conditioned Media

• Published in: Biological & pharmaceutical bulletin Vol. 18; no. 1; pp. 5 - 8
• Main Authors: IKAWA, Masahito, YING, Xing, TANAKA, Keiichi, OHASHI, Kazutomo, SAJI, Fumitaka, OKABE, Masaru
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 01.01.1995; Maruzen; Japan Science and Technology Agency
• Subjects: Acrosome - physiology; Animals; Biological and medical sciences; Cells, Cultured; Cricetinae; Culture Media, Conditioned; Female; Fertilization - physiology; Flow Cytometry; Fundamental and applied biological sciences. Psychology; fusion; hamster egg; human sperm; Humans; In Vitro Techniques; Male; Mammalian male genital system; Mesocricetus; Morphology. Physiology; Ovum - metabolism; Ovum - physiology; Ovum - ultrastructure; Protein Biosynthesis; sperm penetration assay; Spermatozoa - metabolism; Spermatozoa - physiology; Spermatozoa - ultrastructure; Vertebrates: reproduction; Zona Pellucida - physiology; Human; Vertebrata; Mammalia; Fertilizing ability; Investigation method; Egg; Semen; Rodentia; Penetration test; In vitro; Hamster
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.18.5

A conditioned medium (CM) was prepared by culturing human sperm at 3.0×106 sperm/ml for 12 h. The fusion index increased significantly when the sperm penetration assay (SPA) was performed with CM (0.6 without CM to 2.0 with CM). The conditioned medium was also effective on the sperm from 11 patients who showed a low or zero value in SPA and who's in vitro fertilization treatment failed. When CM from the sperm of healthy donors was added to these patients'sperm, the fusing ability to zona free hamster eggs was significantly restored.