Emergent and Neglected Equine Filariosis in Egypt: Species Diversity and Host Immune Response

• Published in: Pathogens (Basel) Vol. 11; no. 9; p. 979
• Main Authors: Abo-Aziza, Faten A M, Hendawy, Seham H M, Abdullah, Hend H A M, El Namaky, Amira, Laidoudi, Younes, Mediannikov, Oleg
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27.08.2022; MDPI
• Subjects: Analysis; Biodiversity; Cytokines; Diseases; Equids; Genotype & phenotype; Health problems; Horses; Host-parasite relationships; Immune response; Immune system; Immunoassays; Immunology; Infections; Infectious diseases; Interleukin 10; Interleukin 5; Life Sciences; Localization; Lymphocytes T; Mansonella sp; Nematodes; Parasites; Phylogeny; Setaria digitata; Species diversity; Vector-borne diseases; Vectors (Biology); Zoonoses; zoonotic filariosis; Egypt; Senegal; Algeria; Setaria digitata; equids; Dirofilaria repens; phylogeny; cytokines; Mansonella sp; zoonotic filariosis
• ISSN: 2076-0817; 2076-0817
• DOI: 10.3390/pathogens11090979

Equine filariosis (EF) is a neglected vector-borne disease caused by nematode species belonging to the Onchocercidae and Setariidae families. Aside from their zoonotic potential, some species are responsible for serious health problems in equids worldwide, leading to significant economic difficulties. Here, we molecularly investigated equine blood samples (320 horses and 109 donkeys from Egypt) and four adult worms isolated from the peritoneal cavity of 5 out of the 94 slaughtered donkeys. In addition, quantitative enzyme-linked immunoassays (ELISAs) targeting circulating cytokines were used to identify whether the immunological profile of the infected animals is a Th1 (i.e., INF-gamma as indicator) or Th2 (i.e., IL-5 and IL-10 as indicators) response type. Overall, 13.8% and 0.3% of the donkeys and horses, respectively, were scored as positive for filaroid DNA. The 18S phylogeny revealed the occurrence of three different filaroid species, identified here as ( ) sp., and . Th1 (INF-gamma and IL-5) and Th2 (IL-10) immune response types were identified in equines infected with and ( ) sp., respectively. These results provide new data on the species diversity of EF in Egypt and extend knowledge of the downregulation of the protective immune response by the potentially zoonotic sp. There is an urgent need to implement control measures to preserve equine health and limit the propagation of these vector-borne filaroids in Egypt.