Prevalence of Nontuberculous Mycobacteria among Extrapulmonary Tuberculosis Cases in Tertiary Care Centers in Northern India

• Published in: BioMed research international Vol. 2015; no. 2015; pp. 1 - 5
• Main Authors: Dhole, T. N., Kumar, M., Kushwaha, R. A. S., Kant, S., Nag, V. L., Maurya, A. K., Singh, A. K.
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2015; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Bacterial Typing Techniques; Deoxyribonucleic acid; Disease; Distribution; DNA; Drug resistance; Female; Genotype; Humans; India - epidemiology; Laboratories; Male; Medical research; Medicine, Experimental; Microbiology; Microscopy; Mortality; Mycobacteria; Mycobacterium; Mycobacterium - classification; Mycobacterium - genetics; Mycobacterium - isolation & purification; Nosocomial infections; Prevalence; Studies; Tertiary Care Centers; Tuberculosis; Tuberculosis - epidemiology; Tuberculosis - genetics; Tuberculosis - microbiology; Vitamin B; India
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2015/465403

The reports of nontuberculous mycobacteria (NTM) associated with extrapulmonary diseases are increasing in tertiary care hospitals. Despite a significant increase in knowledge about NTM infections, they still represent a diagnostic and therapeutic challenge. The aim of this study is to know the prevalence of NTN among extrapulmonary tuberculosis cases in tertiary care centers in Northern India. A total of 227 culture positive isolates from 756 cases were tested for niacin production and catalase assay. BIO-LINE SD Ag MPT64 TB test and final identification and differentiation between MTBC and different species of NTM were further confirmed by GenoType Mycobacterium CM/AS assay. 71 cases (9.3%) were positive for AFB by ZN staining and 227 cases (30.1%) were positive for mycobacteria by culture. Niacin production and catalase activity were negative in 62/227 (27.4%) strains and after using a panel of different biochemicals and final confirmation by GenoType Mycobacterium CM assay. Out of 227 cultures tested, 165 (72.6%) strains were confirmed as M. tuberculosis complex, and 62 (27.4%) were confirmed as NTM. The most common NTM species identified were M. fortuitum 17 (27.5%) and M. intracellulare 13 (20.9%). The rapid identification of NTM species may help in targeted therapy and management of the diseases.