前列腺素E1抑制慢性乙型重型肝炎树突状细胞成熟及CD4~＋/CD8~＋T细胞活性的观察
目的研究前列腺素E1对慢性乙型重型肝炎树突状细胞成熟及CD4＋/CD8＋T细胞活性的影响。方法抽取前列腺素E1治疗10 d前后的慢性乙型重型肝炎患者外周静脉血,密度梯度离心法分离淋巴细胞,贴壁培养获得PBMC,经重组人白细胞介素4（rhIL-4）、重组人粒-巨噬细胞集落刺激因子（rhGM-CSF）、加或不加前列腺素E1刺激培养8 d获得树突细胞（DC）。经流式细胞仪检测DC表达的HLA-DR、CD83、CD86,ELISA测定分泌的IFNγ、IL-12p70。悬浮T细胞部分检测CD4＋/CD8＋T细胞比例及其细胞表面CD69、HLA-DR表达;部分经IL-2培养,用于检测DC体外诱导的T淋巴细...
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| Published in | 临床肝胆病杂志 Vol. 28; no. 3; pp. 212 - 215 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
聊城市人民医院感染科,山东聊城,252000%聊城市人民医院中心实验室,山东聊城,252000%重庆医科大学第二附属医院,重庆,414000
2012
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-5256 |
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| Abstract | 目的研究前列腺素E1对慢性乙型重型肝炎树突状细胞成熟及CD4＋/CD8＋T细胞活性的影响。方法抽取前列腺素E1治疗10 d前后的慢性乙型重型肝炎患者外周静脉血,密度梯度离心法分离淋巴细胞,贴壁培养获得PBMC,经重组人白细胞介素4（rhIL-4）、重组人粒-巨噬细胞集落刺激因子（rhGM-CSF）、加或不加前列腺素E1刺激培养8 d获得树突细胞（DC）。经流式细胞仪检测DC表达的HLA-DR、CD83、CD86,ELISA测定分泌的IFNγ、IL-12p70。悬浮T细胞部分检测CD4＋/CD8＋T细胞比例及其细胞表面CD69、HLA-DR表达;部分经IL-2培养,用于检测DC体外诱导的T淋巴细胞毒活性。结果前列腺素E1治疗慢性乙型重型肝炎,对患者CD4＋/CD8＋T细胞比例无明显影响,可抑制CD4＋/CD8＋T细胞活化分子CD69、HLA-DR的表达;前列腺素E1体外培养的DC低表达HLA-DR、CD83、CD86,分泌IFNγ、IL-12p70均下降,体外诱导的自体淋巴细胞毒活性减弱,与未加用前列腺素E1比较差异有统计学意义（P〈0.01）。结论前列腺素E1治疗慢性乙型重型肝炎,可抑制CD4＋/CD8＋T细胞活化;体外培养可抑制DC成熟,诱导减弱自体淋巴细胞毒活性。 |
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| AbstractList | R512.6; 目的 研究前列腺素E1对慢性乙型重型肝炎树突状细胞成熟及CD4 +/CD8+T细胞活性的影响.方法 抽取前列腺素E1治疗10 d前后的慢性乙型重型肝炎患者外周静脉血,密度梯度离心法分离淋巴细胞,贴壁培养获得PBMC,经重组人白细胞介素4(rhIL-4)、重组人粒-巨噬细胞集落刺激因子(rhGM-CSF)、加或不加前列腺素E1刺激培养8d获得树突细胞(DC).经流式细胞仪检测DC表达的HLA-DR、CD83、CD86,ELISA测定分泌的IFN γ、IL-12p70.悬浮T细胞部分检测CD4 +/CD8+T细胞比例及其细胞表面CD69、HLA-DR表达；部分经IL-2培养,用于检测DC体外诱导的T淋巴细胞毒活性.结果前列腺素E1治疗慢性乙型重型肝炎,对患者CD4 +/CD8+T细胞比例无明显影响,可抑制CD4 +/CD8+T细胞活化分子CD69、HLA-DR的表达；前列腺素E1体外培养的DC低表达HLA-DR、CD83、CD86,分泌IFN γ、IL-12p70均下降,体外诱导的自体淋巴细胞毒活性减弱,与未加用前列腺素E1比较差异有统计学意义(P＜0.01).结论 前列腺素E1治疗慢性乙型重型肝炎,可抑制CD4 +/CD8+T细胞活化；体外培养可抑制DC成熟,诱导减弱自体淋巴细胞毒活性. 目的研究前列腺素E1对慢性乙型重型肝炎树突状细胞成熟及CD4＋/CD8＋T细胞活性的影响。方法抽取前列腺素E1治疗10 d前后的慢性乙型重型肝炎患者外周静脉血,密度梯度离心法分离淋巴细胞,贴壁培养获得PBMC,经重组人白细胞介素4（rhIL-4）、重组人粒-巨噬细胞集落刺激因子（rhGM-CSF）、加或不加前列腺素E1刺激培养8 d获得树突细胞（DC）。经流式细胞仪检测DC表达的HLA-DR、CD83、CD86,ELISA测定分泌的IFNγ、IL-12p70。悬浮T细胞部分检测CD4＋/CD8＋T细胞比例及其细胞表面CD69、HLA-DR表达;部分经IL-2培养,用于检测DC体外诱导的T淋巴细胞毒活性。结果前列腺素E1治疗慢性乙型重型肝炎,对患者CD4＋/CD8＋T细胞比例无明显影响,可抑制CD4＋/CD8＋T细胞活化分子CD69、HLA-DR的表达;前列腺素E1体外培养的DC低表达HLA-DR、CD83、CD86,分泌IFNγ、IL-12p70均下降,体外诱导的自体淋巴细胞毒活性减弱,与未加用前列腺素E1比较差异有统计学意义（P〈0.01）。结论前列腺素E1治疗慢性乙型重型肝炎,可抑制CD4＋/CD8＋T细胞活化;体外培养可抑制DC成熟,诱导减弱自体淋巴细胞毒活性。 |
| Author | 石庆凤 张颖新 宋吉奎 袁建国 李庆方 赵红奎 曾维群 陈敏 |
| AuthorAffiliation | 聊城市人民医院感染科,山东聊城252000 聊城市人民医院中心实验室,山东聊城252000 重庆医科大学第二附属医院,重庆414000 |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | Effect of prostaglandin E1 on the dendritic cells maturation and the activity of CD4~＋/CD8~＋ T cells in chronic severe hepatitis B patients |
| DocumentTitle_FL | Effect of prostaglandin E1 on the dendritic cells maturation and the activity of CD4 +/CD8 + T cells in chronic severe hepatitis B patients |
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| Keywords | 肝炎,乙型,慢性 树突细胞 前列地尔 |
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| Notes | SHI Qing-feng,ZHANG Ying-xin,SONG Ji-kui,et al.（Department of Infection Diseases,Liaocheng Hospital,Liaocheng,Shandong 252000,China） alprostadil; hepatitis B; chronic; dendritic cells Objective To study the maturation of dendritic cells and the activity of CD4＋/CD8＋T cells after prostaglandin E1 treatment in chronic severe hepatitis B patients.Methods PBMC were isolated from fresh peripheral blood of chronic severe hepatitis B patients by Ficoll-Hypaque density gradient centrifugation and cultured with plastic-adherence method.Granulocyte-macrophage colony stimulating factor and interleukin-4 with or without prostaglandin E1 were used to induce the PBMC to obtain dendritic cells for 8 days.FACS was used to analyze the expression of the DC surface markers,including human leukocyte antigen（HLA-DR）,CD83,CD86.The secretion of IFN-γ,IL-12p70 was detected by ELISA.Some of the non-adherent cells were used to analyze the ratio of CD4＋/CD8＋T cells and the expression of CD69 and HLA-DR,the others were incubated with IL-2 |
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| PublicationTitle | 临床肝胆病杂志 |
| PublicationTitleAlternate | Chinese Journal of Clinical Hepatology |
| PublicationTitle_FL | Journal of Clinical Hepatology |
| PublicationYear | 2012 |
| Publisher | 聊城市人民医院感染科,山东聊城,252000%聊城市人民医院中心实验室,山东聊城,252000%重庆医科大学第二附属医院,重庆,414000 |
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| Snippet | 目的研究前列腺素E1对慢性乙型重型肝炎树突状细胞成熟及CD4＋/CD8＋T细胞活性的影响。方法抽取前列腺素E1治疗10... R512.6; 目的 研究前列腺素E1对慢性乙型重型肝炎树突状细胞成熟及CD4 +/CD8+T细胞活性的影响.方法 抽取前列腺素E1治疗10... |
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| SubjectTerms | 前列地尔 树突细胞 肝炎,乙型,慢性 |
| Title | 前列腺素E1抑制慢性乙型重型肝炎树突状细胞成熟及CD4~＋/CD8~＋T细胞活性的观察 |
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