前列腺素E1抑制慢性乙型重型肝炎树突状细胞成熟及CD4~＋/CD8~＋T细胞活性的观察

目的研究前列腺素E1对慢性乙型重型肝炎树突状细胞成熟及CD4＋/CD8＋T细胞活性的影响。方法抽取前列腺素E1治疗10 d前后的慢性乙型重型肝炎患者外周静脉血,密度梯度离心法分离淋巴细胞,贴壁培养获得PBMC,经重组人白细胞介素4（rhIL-4）、重组人粒-巨噬细胞集落刺激因子（rhGM-CSF）、加或不加前列腺素E1刺激培养8 d获得树突细胞（DC）。经流式细胞仪检测DC表达的HLA-DR、CD83、CD86,ELISA测定分泌的IFNγ、IL-12p70。悬浮T细胞部分检测CD4＋/CD8＋T细胞比例及其细胞表面CD69、HLA-DR表达;部分经IL-2培养,用于检测DC体外诱导的T淋巴细...

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Published in临床肝胆病杂志 Vol. 28; no. 3; pp. 212 - 215
Main Author 石庆凤 张颖新 宋吉奎 袁建国 李庆方 赵红奎 曾维群 陈敏
Format Journal Article
LanguageChinese
Published 聊城市人民医院感染科,山东聊城,252000%聊城市人民医院中心实验室,山东聊城,252000%重庆医科大学第二附属医院,重庆,414000 2012
Subjects
前列地尔
树突细胞
肝炎,乙型,慢性
肝炎,乙型,慢性
树突细胞
前列地尔
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ISSN1001-5256

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Summary:目的研究前列腺素E1对慢性乙型重型肝炎树突状细胞成熟及CD4＋/CD8＋T细胞活性的影响。方法抽取前列腺素E1治疗10 d前后的慢性乙型重型肝炎患者外周静脉血,密度梯度离心法分离淋巴细胞,贴壁培养获得PBMC,经重组人白细胞介素4（rhIL-4）、重组人粒-巨噬细胞集落刺激因子（rhGM-CSF）、加或不加前列腺素E1刺激培养8 d获得树突细胞（DC）。经流式细胞仪检测DC表达的HLA-DR、CD83、CD86,ELISA测定分泌的IFNγ、IL-12p70。悬浮T细胞部分检测CD4＋/CD8＋T细胞比例及其细胞表面CD69、HLA-DR表达;部分经IL-2培养,用于检测DC体外诱导的T淋巴细胞毒活性。结果前列腺素E1治疗慢性乙型重型肝炎,对患者CD4＋/CD8＋T细胞比例无明显影响,可抑制CD4＋/CD8＋T细胞活化分子CD69、HLA-DR的表达;前列腺素E1体外培养的DC低表达HLA-DR、CD83、CD86,分泌IFNγ、IL-12p70均下降,体外诱导的自体淋巴细胞毒活性减弱,与未加用前列腺素E1比较差异有统计学意义（P〈0.01）。结论前列腺素E1治疗慢性乙型重型肝炎,可抑制CD4＋/CD8＋T细胞活化;体外培养可抑制DC成熟,诱导减弱自体淋巴细胞毒活性。
Bibliography:SHI Qing-feng,ZHANG Ying-xin,SONG Ji-kui,et al.（Department of Infection Diseases,Liaocheng Hospital,Liaocheng,Shandong 252000,China）
alprostadil; hepatitis B; chronic; dendritic cells
Objective To study the maturation of dendritic cells and the activity of CD4＋/CD8＋T cells after prostaglandin E1 treatment in chronic severe hepatitis B patients.Methods PBMC were isolated from fresh peripheral blood of chronic severe hepatitis B patients by Ficoll-Hypaque density gradient centrifugation and cultured with plastic-adherence method.Granulocyte-macrophage colony stimulating factor and interleukin-4 with or without prostaglandin E1 were used to induce the PBMC to obtain dendritic cells for 8 days.FACS was used to analyze the expression of the DC surface markers,including human leukocyte antigen（HLA-DR）,CD83,CD86.The secretion of IFN-γ,IL-12p70 was detected by ELISA.Some of the non-adherent cells were used to analyze the ratio of CD4＋/CD8＋T cells and the expression of CD69 and HLA-DR,the others were incubated with IL-2
ISSN:1001-5256