美丽异木棉茎腐病病原菌分离与鉴定

从苗圃地采集美丽异木棉茎腐病样本,进行组织分离培养,致病性测定,采用形态学结合多基因（核糖体内转录间隔区、β-微管蛋白、几丁质合成酶、肌动蛋白、3-磷酸甘油醛脱氢酶）分子系统学的方法,对分离菌株进行鉴定,发现分离获得的炭疽菌与其他所有胶孢炭疽菌聚在一起,形成一个明显的分支,确定病原菌为胶孢炭疽菌Colletotrichum gloeosporioides Penz。...

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Bibliographic Details
Published in西南农业学报 Vol. 28; no. 2; pp. 580 - 583
Main Author 廖旺姣 朱英芝 邹东霞 吴耀军 黄华艳 黄乃秀
Format Journal Article
LanguageChinese
Published 广西壮族自治区林业科学研究院,国家林业局中南速生材繁育实验室,广西优良用材林资源培育重点实验室,广西南宁530002 2015
Subjects
病原菌鉴定
茎腐病：胶孢炭疽菌
Pathogen identification
病原菌鉴定
胶孢炭疽菌
Colletotrichum gloeosporioides
Stem rot
茎腐病
Online AccessGet full text
ISSN1001-4829
DOI10.16213/j.cnki.scjas.2015.02.024

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Summary:从苗圃地采集美丽异木棉茎腐病样本,进行组织分离培养,致病性测定,采用形态学结合多基因（核糖体内转录间隔区、β-微管蛋白、几丁质合成酶、肌动蛋白、3-磷酸甘油醛脱氢酶）分子系统学的方法,对分离菌株进行鉴定,发现分离获得的炭疽菌与其他所有胶孢炭疽菌聚在一起,形成一个明显的分支,确定病原菌为胶孢炭疽菌Colletotrichum gloeosporioides Penz。
Bibliography:Stem rot Colletotrichum gloeosporioides Pathogen identification
51-1213/S
A serious stem rot of Chorisia speciosa was found in tree nursery. The pathogen was isolated and incubated,pathogenicity tested.and was identified as Colletotrichum gloeosporioides Penz. through morphological and multtilous phylogenetic analysis（ internal transcribed spaces,ITS; β-tubulin,TUB2; chitin synthase A,CHSⅠ; actin,ACT; glyceraldehydes-3-phosphate dehydrogenase,GPDH）.
LIAO Wang-jiao, ZHU Ying-zhi, ZOU Dong-xia, WU Yao-jun, HUANG Hua-yan, HUANG Nai-xiu （ Guangxi Zhuang Autonomous Region Forestry Research Institute, Key Laboratory of Central South Fast-growing Timber Cultivation of Forestry Ministry of China, Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Guangxi Nanning 530002, China）
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2015.02.024