肝细胞肝癌肿瘤微环境中功能耗竭CD8^＋T细胞脂代谢特征的观察

目的：探讨肝细胞肝癌（hepatocellular carcinoma,HCC）肿瘤微环境中CD8^＋T细胞耗竭与脂代谢的关系。方法：利用流式细胞分析、实时细胞能量代谢检测仪、实时PCR等方法检测HCC和癌旁组织中CD8^＋T细胞脂代谢及功能相关蛋白或基因的表达;在此基础上分析其功能和脂代谢的关系。结果：HCC浸润的CD8^＋T细胞具有以下特征：功能耗竭（干扰素-γ减少、程序性死亡分子-1表达升高）;多个脂肪酸合成酶表达下调,脂肪酸含量下降;线粒体结构、功能受损（P〈0.05）。结论：HCC中浸润的功能耗竭CD8^＋T细胞具有脂肪酸代谢异常特征。...

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Published in中国临床医学 Vol. 24; no. 4; pp. 514 - 518
Main Author 沈琦 卓乐盈 刘芳铭 张苗苗 王晗 刘卫仁 武多娇
Format Journal Article
LanguageChinese
Published 复旦大学附属中山医院实验研究中心,上海,200032%温州医科大学,温州,325035 2017
Subjects
CD8^＋T细胞
代谢
功能耗竭
微环境
肝细胞肝癌
microenvironment
CD8+T cells
functional exhaustion
代谢
功能耗竭
微环境
metabolism
CD8+T细胞
肝细胞肝癌
hepatocellular carcinoma
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ISSN1008-6358
DOI10.12025/j.issn.1008-6358.2017.20170621

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Summary:目的：探讨肝细胞肝癌（hepatocellular carcinoma,HCC）肿瘤微环境中CD8^＋T细胞耗竭与脂代谢的关系。方法：利用流式细胞分析、实时细胞能量代谢检测仪、实时PCR等方法检测HCC和癌旁组织中CD8^＋T细胞脂代谢及功能相关蛋白或基因的表达;在此基础上分析其功能和脂代谢的关系。结果：HCC浸润的CD8^＋T细胞具有以下特征：功能耗竭（干扰素-γ减少、程序性死亡分子-1表达升高）;多个脂肪酸合成酶表达下调,脂肪酸含量下降;线粒体结构、功能受损（P〈0.05）。结论：HCC中浸润的功能耗竭CD8^＋T细胞具有脂肪酸代谢异常特征。
Bibliography:31-1794/R
Objective：To explore the lipid metabolic characteristics of functionally exhausted CD8^＋ T cells in tumor microenvironment of hepatocellular carcinoma（HCC）.Methods：By using flow cytometry analysis and real-time PCR to detect the functions and lipid metabolism-related proteins and genes expression of CD8^＋ T cells in HCC and peri-tumor tissues,then the relationship between function and lipid metabolism was analyzed.Results：The infiltrated CD8^＋ T cells in HCC have the following characteristics：functional exhaustion（decreased interferon-γsecretion and up regulated programmed death molecule-1 expression）;down regulated expression of multiple fatty acid synthases,and the decreased content of intracellular fatty acids;impaired mitochondrial structure and function（P0.05）.Conclusions：Fatty acid metabolism abnormalities are the characteristics of exhausted CD8^＋ T cell infiltrated in HCC.
hepatocellular carcinoma; CD8^＋ T cells ; metabolism; functional exhaustion; microenvironment
SHEN Qi1 , ZHUO Le-ying2 , LI
ISSN:1008-6358
DOI:10.12025/j.issn.1008-6358.2017.20170621