Interaction of aspirin (acetylsalicylic acid) with lipid membranes

We studied the interaction of Aspirin (acetylsalicylic acid) with lipid membranes using x-ray diffraction for bilayers containing up to 50 mol% of aspirin. From 2D x-ray intensity maps that cover large areas of reciprocal space we determined the position of the ASA molecules in the phospholipid bila...

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Published inPloS one Vol. 7; no. 4; p. e34357
Main Authors Barrett, Matthew A, Zheng, Songbo, Roshankar, Golnaz, Alsop, Richard J, Belanger, Randy K R, Huynh, Chris, Kučerka, Norbert, Rheinstädter, Maikel C
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 17.04.2012
Public Library of Science (PLoS)
Subjects
Acetylsalicylic acid
Acids
Aircraft
Alcohol
Aspirin
Aspirin - chemistry
Astronomy
Biochemistry
Biology
Cell Membrane - chemistry
Cholesterol
Cholesterol - chemistry
Crystal structure
Dimyristoylphosphatidylcholine - chemistry
Electrons
Hydration
Hydrophobic and Hydrophilic Interactions
Hydrophobicity
Lipid bilayers
Lipid Bilayers - chemistry
Lipid membranes
Lipids
Medicine
Membranes
Phosphocholine
Phospholipids
Physical chemistry
Physics
Salicylates
Silicon wafers
Sterols
X-Ray Diffraction
Ontario Canada
Canada
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Summary:We studied the interaction of Aspirin (acetylsalicylic acid) with lipid membranes using x-ray diffraction for bilayers containing up to 50 mol% of aspirin. From 2D x-ray intensity maps that cover large areas of reciprocal space we determined the position of the ASA molecules in the phospholipid bilayers and the molecular arrangement of the molecules in the plane of the membranes. We present direct experimental evidence that ASA molecules participate in saturated lipid bilayers of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) and preferably reside in the head group region of the membrane. Up to 50 mol% ASA molecules can be dissolved in this type of bilayer before the lateral membrane organization is disturbed and the membranes are found to form an ordered, 2D crystal-like structure. Furthermore, ASA and cholesterol were found to co-exist in saturated lipid bilayers, with the ASA molecules residing in the head group region and the cholesterol molecules participating in the hydrophobic membrane core.
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Conceived and designed the experiments: MCR. Performed the experiments: MAB SZ GR RJA RKRB CH. Analyzed the data: MAB NK MCR. Contributed reagents/materials/analysis tools: MAB SZ GR RJA RKRB CH. Wrote the paper: MAB MCR.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0034357