In vivo efficacy of doripenem (DRPM) against Pseudomonas aeruginosa in murine chronic respiratory tract infection model

• Published in: Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy Vol. 17; no. 3; pp. 318 - 321
• Main Authors: Araki, Nobuko, Yanagihara, Katsunori, Morinaga, Yoshitomo, Yamada, Koichi, Yamada, Yasuaki, Kamihira, Shimeru, Kohno, Shigeru
• Format: Journal Article
• Language: English
• Published: Japan Elsevier Ltd 01.06.2011; Springer Japan
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Carbapenem; Carbapenems - pharmacology; Chronic respiratory infection; Doripenem; Hematology, Oncology and Palliative Medicine; Infectious Diseases; Lung; Male; Medical Microbiology; Medicine; Medicine & Public Health; Mice; Mouse model; Original Article; Pseudomonas aeruginosa; Pseudomonas aeruginosa - drug effects; Pseudomonas Infections - drug therapy; Pseudomonas Infections - microbiology; Pseudomonas Infections - pathology; Respiratory Tract Infections - drug therapy; Respiratory Tract Infections - microbiology; Respiratory Tract Infections - pathology; Specific Pathogen-Free Organisms; Thienamycins - pharmacology; Virology; Carbapenem; Pseudomonas aeruginosa; Doripenem; Mouse model; Lung; Chronic respiratory infection
• ISSN: 1341-321X; 1437-7780
• DOI: 10.1007/s10156-010-0166-1

Abstract Doripenem is a carbapenem antibiotic with broad-spectrum coverage of gram-positive and gram-negative bacteria, including Pseudomonas aeruginosa , and is considered to be as effective as meropenem. The in vivo activity of doripenem was thus compared with that of meropenem in a chronic lower respiratory P. aeruginosa infection mouse model. The number of viable bacteria in the lungs of mice after treatment with doripenem, meropenem, and saline was 2.01 ± 0.69, 2.03 ± 0.48, and 3.90 ± 1.40 log10  CFU/lung, respectively. The number of viable bacteria in the lungs of mice treated with doripenem and meropenem was significantly lower than that in lungs of controls. Histopathological examination of lung specimens from the control group revealed promotion of the inflammatory response in chronic bronchial infection. However, the groups treated with doripenem and meropenem showed weaker inflammatory responses. These results suggest that doripenem treatment is effective against chronic airway infection with P. aeruginosa.