Expression profiling of selected glutathione transferase genes in Zea mays (L.) seedlings infested with cereal aphids

• Published in: PloS one Vol. 9; no. 11; p. e111863
• Main Authors: Sytykiewicz, Hubert, Chrzanowski, Grzegorz, Czerniewicz, Paweł, Sprawka, Iwona, Łukasik, Iwona, Goławska, Sylwia, Sempruch, Cezary
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 03.11.2014; Public Library of Science (PLoS)
• Subjects: Analysis; Animals; Anions; Aphids; Binding sites; Biochemistry; Biology and Life Sciences; Birds; Corn; Cultivars; Enzymes; Gene expression; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Genes; Glutathione; Glutathione transferase; Glutathione Transferase - biosynthesis; Grain; Hemiptera; Herbicides; Humanities; Infestation; Insects; Metabolism; Molecular biology; Oxidative stress; Plant Diseases - parasitology; Plant Proteins - biosynthesis; Radicals; Rhopalosiphum padi; Seedlings; Seedlings - enzymology; Seedlings - parasitology; Seeds; Signal transduction; Sitobion avenae; Superoxide; Superoxides; Thiols; Tissues; Transcription; Zea mays; Zea mays - enzymology; Zea mays - parasitology; Poland
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0111863

The purpose of this report was to evaluate the expression patterns of selected glutathione transferase genes (gst1, gst18, gst23 and gst24) in the tissues of two maize (Zea mays L.) varieties (relatively resistant Ambrozja and susceptible Tasty Sweet) that were colonized with oligophagous bird cherry-oat aphid (Rhopalosiphum padi L.) or monophagous grain aphid (Sitobion avenae L.). Simultaneously, insect-triggered generation of superoxide anion radicals (O2•-) in infested Z. mays plants was monitored. Quantified parameters were measured at 1, 2, 4, 8, 24, 48 and 72 h post-initial aphid infestation (hpi) in relation to the non-infested control seedlings. Significant increases in gst transcript amounts were recorded in aphid-stressed plants in comparison to the control seedlings. Maximal enhancement in the expression of the gst genes in aphid-attacked maize plants was found at 8 hpi (gst23) or 24 hpi (gst1, gst18 and gst24) compared to the control. Investigated Z. mays cultivars formed excessive superoxide anion radicals in response to insect treatments, and the highest overproduction of O2•- was noted 4 or 8 h after infestation, depending on the aphid treatment and maize genotype. Importantly, the Ambrozja variety could be characterized as having more profound increments in the levels of gst transcript abundance and O2•- generation in comparison with the Tasty Sweet genotype.