Closed-Tube Barcoding

• Published in: Genome Vol. 59; no. 11; pp. 1049 - 1061
• Main Authors: Sirianni, Nicky M, Yuan, Huijun, Rice, John E, Kaufman, Ronit S, Deng, John, Fulton, Chandler, Wangh, Lawrence J
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.11.2016; Canadian Science Publishing NRC Research Press
• Subjects: Animals; Bacterial Proteins - genetics; Biodiversity; Cluster Analysis; Computational Biology - methods; Deoxyribonucleic acid; DNA; DNA Barcoding, Taxonomic - methods; DNA sequencing; DNA-Directed RNA Polymerases - genetics; Electron Transport Complex IV - genetics; Genetic recombination; High-Throughput Nucleotide Sequencing; Humans; Methods; Nucleotide sequencing; Polymerase chain reaction; Polymerase Chain Reaction - methods; Workflow; Closed-Tube Barcoding; signatures en fluorescence; codage à barres en tube fermé (« Closed-Tube Barcoding »); Pan-phylum probes; Sondes noir et blanc (« Lights-On/Lights-Off probes »); fluorescent signatures; Lights-On/Lights-Off probes; sondes pan-spécifiques; LATE-PCR
• ISSN: 0831-2796; 1480-3321
• DOI: 10.1139/gen-2016-0026

Here, we present a new approach for increasing the rate and lowering the cost of identifying, cataloging, and monitoring global biodiversity. These advances, which we call Closed-Tube Barcoding, are one application of a suite of proven PCR-based technologies invented in our laboratory. Closed-Tube Barcoding builds on and aims to enhance the profoundly important efforts of the International Barcode of Life initiative. Closed-Tube Barcoding promises to be particularly useful when large numbers of small or rare specimens need to be screened and characterized at an affordable price. This approach is also well suited for automation and for use in portable devices.