Upregulation of Pluripotency Markers in Adipose Tissue-Derived Stem Cells by miR-302 and Leukemia Inhibitory Factor

• Published in: BioMed research international Vol. 2014; no. 2014; pp. 1 - 10
• Main Authors: Taha, Masoumeh Fakhr, Javeri, Arash, Rohban, Sara, Mowla, Seyed Javad
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01.01.2014; Hindawi Publishing Corporation; John Wiley & Sons, Inc
• Subjects: Adipose Tissue - metabolism; Adipose tissues; Animals; Biomarkers - metabolism; Biomedical research; Biotechnology; Body fat; Care and treatment; Development and progression; Embryonic stem cells; Gene expression; Genetic aspects; Genetic engineering; Leukemia; Leukemia Inhibitory Factor - genetics; Leukemia Inhibitory Factor - metabolism; Mesenchymal Stromal Cells - metabolism; Mice; Mice, Inbred BALB C; MicroRNAs - genetics; MicroRNAs - metabolism; Octamer Transcription Factor-3 - genetics; Octamer Transcription Factor-3 - metabolism; Physiological aspects; Polymerase chain reaction; Proteins; Risk factors; RNA, Messenger - genetics; SOXB1 Transcription Factors - genetics; SOXB1 Transcription Factors - metabolism; Stem cells; Studies; Up-Regulation - genetics; United States
• ISSN: 2314-6133; 2314-6141; 2314-6141
• DOI: 10.1155/2014/941486

The expression pattern of pluripotency markers in adipose tissue-derived stem cells (ADSCs) is a subject of controversy. Moreover, there is no data about the signaling molecules that regulate these markers in ADSCs. In the present study, we studied the roles of leukemia inhibitory factor (LIF) and miR-302 in this regard. Freshly isolated mouse ADSCs expressed hematopoietic, mesenchymal, and pluripotency markers. One day after plating, ADSCs expressed OCT4 and Sox2 proteins. After three passages, the expression of hematopoietic and pluripotency markers decreased, while the expression of mesenchymal stem cell markers exhibited a striking rise. Both supplementation of culture media with LIF and transfection of the ADSCs with miR-302 family upregulated the expression levels of OCT4, Nanog, and Sox2 mRNAs. These findings showed that mouse adipose tissue contains a population of cells with molecular resemblance to embryonic stem cells, and LIF and miR-302 family positively affect the expression of pluripotency markers.