Effects of day length- and temperature-regulated genes on annual transcriptome dynamics in Japanese cedar (Cryptomeria japonica D. Don), a gymnosperm indeterminate species

• Published in: PloS one Vol. 15; no. 3; p. e0229843
• Main Authors: Nose, Mine, Kurita, Manabu, Tamura, Miho, Matsushita, Michinari, Hiraoka, Yuichiro, Iki, Taiichi, Hanaoka, So, Mishima, Kentaro, Tsubomura, Miyoko, Watanabe, Atsushi
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 09.03.2020; Public Library of Science (PLoS)
• Subjects: Analysis; Biology and Life Sciences; Cedar; Climate change; Cryptomeria - genetics; Cryptomeria japonica; Cycadopsida - genetics; Data analysis; Day length; DNA microarrays; Environmental conditions; Environmental quality; Environmental science; Fisheries; Forest products; Forestry; Gene expression; Gene Expression Regulation, Plant; Gene regulation; Genes; Genes, Plant - genetics; Genes, Regulator; Instrument industry (Equipment); Low temperature; Network analysis; Physical Sciences; Principal components analysis; Research and Analysis Methods; Seasons; Species; Target recognition; Temperature; Temperature effects; Transcription; Transcriptome; Trees; Trees - genetics; Trees - metabolism; Uniqueness; United States; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0229843

Seasonal phenomena in plants are primarily affected by day length and temperature. The shoot transcriptomes of trees grown in the field and a controlled-environment chamber were compared to characterize genes that control annual rhythms and the effects of day length- and temperature-regulated genes in the gymnosperm Japanese cedar (Cryptomeria japonica D. Don), which exhibits seasonally indeterminate growth. Annual transcriptome dynamics were clearly demonstrated by principal component analysis using microarray data obtained under field-grown conditions. Analysis of microarray data from trees grown in a controlled chamber identified 2,314 targets exhibiting significantly different expression patterns under short-day (SD) and long-day conditions, and 2,045 targets exhibited significantly different expression patterns at 15°C (LT; low temperature) versus 25°C. Interestingly, although growth was suppressed under both SD and LT conditions, approximately 80% of the SD- and LT-regulated targets differed, suggesting that each factor plays a unique role in the annual cycle. The top 1,000 up-regulated targets in the growth/dormant period in the field coincided with more than 50% of the SD- and LT-regulated targets, and gene co-expression network analysis of the annual transcriptome indicated a close relationship between the SD- and LT-regulated targets. These results indicate that the respective effects of day length and temperature interact to control annual transcriptome dynamics. Well-known upstream genes of signaling pathways responsive to environmental conditions, such as the core clock (LHY/CjLHYb and CCA1/CjLHYa) and PEBP family (MFT) genes, exhibited unique expression patterns in Japanese cedar compared with previous reports in other species, suggesting that these genes control differences in seasonal regulation mechanisms between species. The results of this study provide new insights into seasonal regulation of transcription in Japanese cedar.