Optical triggered seizures using a caged 4-Aminopyridine
Animal models of epilepsy are critical not only for understanding the fundamental mechanism of epilepsy but also for testing the efficacy of new antiepileptic drugs and novel therapeutic interventions. Photorelease of caged molecules is widely used in biological research to control pharmacologic eve...
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Published in | Frontiers in neuroscience Vol. 9; p. 25 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Research Foundation
04.02.2015
Frontiers Media S.A |
Subjects | |
Online Access | Get full text |
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Summary: | Animal models of epilepsy are critical not only for understanding the fundamental mechanism of epilepsy but also for testing the efficacy of new antiepileptic drugs and novel therapeutic interventions. Photorelease of caged molecules is widely used in biological research to control pharmacologic events with high spatio-temporal resolution. We developed a technique for in vivo optical triggering of neocortical seizures using a novel caged compound based on ruthenium photochemistry (RuBi-4AP). Epileptiform events in mouse cortex were induced with blue light in both whole brain and focal illumination. Multi-electrode array recording and optical techniques were used to characterize the propagation of these epileptic events, including interictal spikes, polyspikes, and ictal discharges. These results demonstrate a novel optically-triggered seizure model, with high spatio-temporal control, that could have widespread application in the investigation of ictal onset, propagation and to develop novel light-based therapeutic interventions. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Anna W. Roe, Vanderbilt University, USA; Jean-Christophe Sandoz, Centre National de la Recherche Scientifique, France This article was submitted to Brain Imaging Methods, a section of the journal Frontiers in Neuroscience. Edited by: Jorge J. Riera, Florida International University, USA |
ISSN: | 1662-4548 1662-453X 1662-453X |
DOI: | 10.3389/fnins.2015.00025 |